1.14.16.1: phenylalanine 4-monooxygenase
This is an abbreviated version!
For detailed information about phenylalanine 4-monooxygenase, go to the full flat file.
Word Map on EC 1.14.16.1
-
1.14.16.1
-
phenylketonuria
-
hyperphenylalaninemia
-
bh4
-
error
-
pterins
-
inborn
-
children
-
hydroxylases
-
neurotransmitter
-
province
-
tetrahydropterins
-
counsel
-
intellectual
-
dopamine
-
l-tyrosine
-
genotype-phenotype
-
prenatal
-
serotonin
-
dihydropteridine
-
caucasian
-
catecholamine
-
hepatocytes
-
sepiapterin
-
genotype-based
-
quinonoid
-
non-heme
-
chromobacterium
-
neopterin
-
ligation-dependent
-
dihydrochloride
-
neuropsychological
-
lysolecithin
-
lifelong
-
phenylpyruvate
-
dopa
-
cyclohydrolase
-
molecular biology
-
rflps
-
p-chlorophenylalanine
-
dihydrobiopterin
-
hypopigmentation
-
s-oxidation
-
pteridine
-
violaceum
- 1.14.16.1
- phenylketonuria
- hyperphenylalaninemia
- bh4
- error
- pterins
-
inborn
- children
- hydroxylases
-
neurotransmitter
-
province
- tetrahydropterins
-
counsel
-
intellectual
- dopamine
- l-tyrosine
-
genotype-phenotype
-
prenatal
- serotonin
- dihydropteridine
-
caucasian
- catecholamine
- hepatocytes
- sepiapterin
-
genotype-based
-
quinonoid
-
non-heme
-
chromobacterium
- neopterin
-
ligation-dependent
- dihydrochloride
-
neuropsychological
- lysolecithin
-
lifelong
- phenylpyruvate
- dopa
-
cyclohydrolase
- molecular biology
-
rflps
- p-chlorophenylalanine
- dihydrobiopterin
-
hypopigmentation
-
s-oxidation
- pteridine
- violaceum
Reaction
Synonyms
cePAH, DicPAH, EC 1.14.3.1, EC 1.99.1.2, HPAH, L-phenylalanine 4-hydroxylase, oxygenase, phenylalanine 4-mono-, P4H, PAH, PheH, phenylalaninase, phenylalanine 4-hydroxylase, phenylalanine hydroxylase, phenylalanine monooxygenase, PheOH, phhA
ECTree
1.14.16.1 phenylalanine 4-monooxygenaseAdvanced search results
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results (KM Value
KM Value on EC 1.14.16.1 - phenylalanine 4-monooxygenase
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KM VALUE [mM] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
8.3
(S)-carboxymethyl-L-cysteine
wild type enzyme, in 50 mM potassium phosphate buffer, pH 6.8, at 37°C
20.3
(S)-methyl-L-cysteine
wild type enzyme, in 50 mM potassium phosphate buffer, pH 6.8, at 37°C
0.054
2-amino-4-hydroxy-6,7-dimethyltetrahydropteridine
-
-
29.8
N-acetyl-(S)-carboxymethyl-L-cysteine
wild type enzyme, in 50 mM potassium phosphate buffer, pH 6.8, at 37°C
32.1
N-acetyl-(S)-methyl-L-cysteine
wild type enzyme, in 50 mM potassium phosphate buffer, pH 6.8, at 37°C
0.025
(6R)-5,6,7,8-tetrahydrobiopterin
-
recombinant enzyme, phenylalanine-activated
0.008
(6R)-L-erythro-5,6,7,8-tetrahydrobiopterin
-
wild-type, pH 7.3, 25°C, measurement by continuous PAH activity assay (direct in-well fluorescence detection): Vmax: 2.277 micromol L-Tyr/min/mg (without L-Phe preincubation)
0.024
(6R)-L-erythro-5,6,7,8-tetrahydrobiopterin
-
wild-type, pH 7.3, 25°C, measurement by standard discontinuous PAH activity assay (HPLC and fluorimetric detection): Vmax: 3.425 micromol L-Tyr/min/mg (with L-Phe preincubation)
0.027
(6R)-L-erythro-5,6,7,8-tetrahydrobiopterin
mutant enzyme R155H, in 100 mM Na-HEPES buffer, pH 7.0, at 25°C
0.029
(6R)-L-erythro-5,6,7,8-tetrahydrobiopterin
-
wild-type, pH 7.0, 25°C
0.03
(6R)-L-erythro-5,6,7,8-tetrahydrobiopterin
mutant enzyme P416Q, in 100 mM Na-HEPES buffer, pH 7.0, at 25°C
0.033
(6R)-L-erythro-5,6,7,8-tetrahydrobiopterin
wild type enzyme, in 100 mM Na-HEPES buffer, pH 7.0, at 25°C
0.035
(6R)-L-erythro-5,6,7,8-tetrahydrobiopterin
mutant enzyme R408W, in 100 mM Na-HEPES buffer, pH 7.0, at 25°C
0.04
(6R)-L-erythro-5,6,7,8-tetrahydrobiopterin
mutant enzyme L348V, in 100 mM Na-HEPES buffer, pH 7.0, at 25°C
0.053
(6R)-L-erythro-5,6,7,8-tetrahydrobiopterin
mutant enzyme D143G, in 100 mM Na-HEPES buffer, pH 7.0, at 25°C
0.094
(6R)-L-erythro-5,6,7,8-tetrahydrobiopterin
-
mutant Q215K/N216Y, pH 7.0, 25°C
0.022
5,6,7,8-tetrahydrobiopterin
mutant enzyme Y414C, using L-phenylalanine as cosubstrate
0.0231
5,6,7,8-tetrahydrobiopterin
-
using L-phenylalanine as cosubstrate, in pooled hepatic cytosolic enzyme fraction, at 37°C
0.02333
5,6,7,8-tetrahydrobiopterin
-
using L-phenylalanine as cosubstrate, in pooled hepatic cytosolic enzyme fraction activated with 1 mM lysophosphatidylcholine, at 37°C
0.024
5,6,7,8-tetrahydrobiopterin
mutant enzyme V388M, using L-phenylalanine as cosubstrate
0.026
5,6,7,8-tetrahydrobiopterin
wild type enzyme, using L-phenylalanine as cosubstrate
0.027
5,6,7,8-tetrahydrobiopterin
mutant enzyme R261Q, using L-phenylalanine as cosubstrate
0.032
5,6,7,8-tetrahydrobiopterin
mutant enzyme R68S, using L-phenylalanine as cosubstrate
0.035
5,6,7,8-tetrahydrobiopterin
mutant enzyme W180F, at 30°C, in 50 mM HEPES-NaOH buffer (pH 7.5)
0.04
5,6,7,8-tetrahydrobiopterin
mutant enzyme I65T, using L-phenylalanine as cosubstrate
0.0728
5,6,7,8-tetrahydrobiopterin
wild type enzyme, using S-carboxymethyl-L-cysteine as cosubstrate
0.07312
5,6,7,8-tetrahydrobiopterin
-
using S-carboxymethyl-L-cysteine as cosubstrate, in pooled hepatic cytosolic enzyme fraction activated with 1 mM lysophosphatidylcholine, at 37°C
0.07812
5,6,7,8-tetrahydrobiopterin
-
using S-carboxymethyl-L-cysteine as cosubstrate, in pooled hepatic cytosolic enzyme fraction, at 37°C
0.086
5,6,7,8-tetrahydrobiopterin
mutant enzyme L101Y/W180F, at 30°C, in 50 mM HEPES-NaOH buffer (pH 7.5)
0.099
5,6,7,8-tetrahydrobiopterin
mutant enzyme L101Y, at 30°C, in 50 mM HEPES-NaOH buffer (pH 7.5)
0.14
5,6,7,8-tetrahydrobiopterin
mutant enzyme R68S, using S-carboxymethyl-L-cysteine as cosubstrate
0.14
5,6,7,8-tetrahydrobiopterin
wild type enzyme, at 30°C, in 50 mM HEPES-NaOH buffer (pH 7.5)
0.143
5,6,7,8-tetrahydrobiopterin
mutant enzyme Y414C, using S-carboxymethyl-L-cysteine as cosubstrate
0.146
5,6,7,8-tetrahydrobiopterin
mutant enzyme V388M, using S-carboxymethyl-L-cysteine as cosubstrate
0.15
5,6,7,8-tetrahydrobiopterin
mutant enzyme I65T, using S-carboxymethyl-L-cysteine as cosubstrate
0.155
5,6,7,8-tetrahydrobiopterin
mutant enzyme R261Q, using S-carboxymethyl-L-cysteine as cosubstrate
0.0344
6,7-dimethyl-5,6,7,8-tetrahydropterin
-
enzyme form II, substrate L-phenylalanine
0.0444
6,7-dimethyl-5,6,7,8-tetrahydropterin
-
enzyme form I, substrate L-phenylalanine
0.152
6,7-dimethyl-5,6,7,8-tetrahydropterin
wild type enzyme, at pH 7.4 and 20°C
0.236
6,7-dimethyl-5,6,7,8-tetrahydropterin
mutant enzyme D139N, at pH 7.4 and 20°C
0.254
6,7-dimethyl-5,6,7,8-tetrahydropterin
mutant enzyme D139N, at pH 7.4 and 20°C
0.262
6,7-dimethyl-5,6,7,8-tetrahydropterin
mutant enzyme D139N, at pH 7.4 and 20°C
0.105
6,7-dimethyltetrahydrobiopterin
-
chymotrypsin activated liver enzyme
0.105
6,7-dimethyltetrahydrobiopterin
-
chymotrypsin-activated enzyme, at pH 6.8 and 25°C
0.037
6-Methyl-5,6,7,8-tetrahydropterin
-
enzyme form I, substrate L-phenylalanine
0.0455
6-Methyl-5,6,7,8-tetrahydropterin
-
enzyme form II, substrate L-phenylalanine
0.063
6-methyl-tetrahydrobiopterin
-
pH 7.0, 25°C, recombinant mutant DELTA 117PheH V379D
0.083
6-methyl-tetrahydrobiopterin
-
pH 7.0, 25°C, recombinant mutant DELTA 117PheH
0.073
6-methyltetrahydropterin
-
maltose-binding-protein phenylalanine fusion protein
0.085
6-methyltetrahydropterin
-
truncated enzyme containing C-terminal 334 amino acids
0.1
L-Phe
-
allelic combination wild-type/mutant I65T, Vmax: 1 micromol/min/mg, pH 6.8, 37°C
0.1
L-Phe
-
allelic combination wild-type/mutant R68S, Vmax: 1 micromol/min/mg, pH 6.8, 37°C
0.1
L-Phe
-
allelic combination wild-type/wild-type, Vmax: 3 micromol/min/mg, pH 6.8, 37°C
0.2
L-Phe
-
allelic combination wild-type/mutant Y414C, Vmax: 1 micromol/min/mg, pH 6.8, 37°C
0.9
L-Phe
-
allelic combination wild-type/mutant V388M, Vmax: 0.9 micromol/min/mg, pH 6.8, 37°C
1
L-Phe
-
allelic combination wild-type/mutant R158Q, Vmax: 0.9 micromol/min/mg, pH 6.8, 37°C
1
L-Phe
-
allelic combination wild-type/mutant R261Q, Vmax: 0.9 micromol/min/mg, pH 6.8, 37°C
2
L-Phe
-
allelic combination wild-type/mutant I174T, Vmax: 0.7 micromol/min/mg, pH 6.8, 37°C
3
L-Phe
-
allelic combination wild-type/mutant R408W, Vmax: 0.7 micromol/min/mg, pH 6.8, 37°C
0.022
L-phenylalanine
mutant enzyme Y414C, in 50 mM potassium phosphate buffer (pH 6.8), at 37°C
0.024
L-phenylalanine
mutant enzyme V388M, in 50 mM potassium phosphate buffer (pH 6.8), at 37°C
0.026
L-phenylalanine
wild type enzyme, in 50 mM potassium phosphate buffer (pH 6.8), at 37°C
0.027
L-phenylalanine
mutant enzyme R261Q, in 50 mM potassium phosphate buffer (pH 6.8), at 37°C
0.032
L-phenylalanine
mutant enzyme R68S, in 50 mM potassium phosphate buffer (pH 6.8), at 37°C
0.033
L-phenylalanine
mutant enzyme W180F, at 30°C, in 50 mM HEPES-NaOH buffer (pH 7.5)
0.04
L-phenylalanine
mutant enzyme I65T, in 50 mM potassium phosphate buffer (pH 6.8), at 37°C
0.05
L-phenylalanine
-
post-translational activation of human cDNA-expressed PAH, activator: NO (nitric oxide) (0.01 mM), Vmax: 33.05 nmoles/min/mg, pH 6.8, 37°C
0.06
L-phenylalanine
-
post-translational activation of human cDNA-expressed PAH, activator: ONOO- (peroxynitrite)(1 mM), Vmax: 32.65 nmoles/min/mg, pH 6.8, 37°C
0.07
L-phenylalanine
-
post-translational activation of human cDNA-expressed PAH, activator: HO (hydroxyl radical) (0.01 mM), Vmax: 32.3 nmoles/min/mg, pH 6.8, 37°C
0.09
L-phenylalanine
-
native liver enzyme in crude extract, cofactor 6-methyltetrahydropterin
0.111
L-phenylalanine
wild type enzyme, at 30°C, in 50 mM HEPES-NaOH buffer (pH 7.5)
0.12
L-phenylalanine
-
pH 7.0, 25°C, with cofactor 6-methyl-tetrahydrobiopterin, recombinant mutant DELTA 117PheH
0.12
L-phenylalanine
-
post-translational activation of human cDNA-expressed PAH, activator: lysophosphatidylcholine (5 mM), Vmax: 3.72 nmoles/min/mg, pH 6.8, 37°C
0.12
L-phenylalanine
-
post-translational activation of human cDNA-expressed PAH, activator: N-ethylmaleimide (50 mM), Vmax: 9.3 nmoles/min/mg, pH 6.8, 37°C
0.1365
L-phenylalanine
mutant enzyme Y155A, in 0.1 M Na-HEPES (pH 7.4) at 30°C
0.137
L-phenylalanine
mutant enzyme D139N, at pH 7.4 and 20°C
0.14
L-phenylalanine
-
post-translational activation of human cDNA-expressed PAH, activator: H2O2 (0.1 mM), Vmax: 28.4 nmoles/min/mg, pH 6.8, 37°C
0.14
L-phenylalanine
-
post-translational activation of human cDNA-expressed PAH, activator: L-Phe (4 mM), Vmax: 1.86 nmoles/min/mg, pH 6.8, 37°C
0.16
L-phenylalanine
-
in pooled hepatic cytosolic enzyme fraction activated with 1 mM lysophosphatidylcholine, at 37°C
0.194
L-phenylalanine
-
cofactor tetrahydrobiopterin, maltose-binding-protein phenylalanine fusion protein
0.225
L-phenylalanine
mutant enzyme D139N, at pH 7.4 and 20°C
0.236
L-phenylalanine
-
cofactor tetrahydrobiopterin, cleaved maltose-binding-protein phenylalanine fusion protein
0.244
L-phenylalanine
wild type enzyme, in 0.1 M Na-HEPES (pH 7.4) at 30°C
0.253
L-phenylalanine
mutant enzyme L101Y/W180F, at 30°C, in 50 mM HEPES-NaOH buffer (pH 7.5)
0.287
L-phenylalanine
-
wild type enzyme, 5 min preincubation with L-phenylalanine, 100 mM Tris-HCl (pH 7.5), at 37°C
0.3 - 0.4
L-phenylalanine
-
native and recombinant enzyme, cofactor 6-methyltetrahydropterin
0.316
L-phenylalanine
mutant enzyme D139N, at pH 7.4 and 20°C
0.318
L-phenylalanine
-
wild-type, pH 7.3, 25°C, measurement by continuous PAH activity assay (direct in-well fluorescence detection): Vmax: 2.53 micromol L-Tyr/min/mg (without L-Phe preincubation), 6.59 micromol L-Tyr/min/mg (with L-Phe preincubation)
0.414
L-phenylalanine
mutant enzyme T254A, in 0.1 M Na-HEPES (pH 7.4) at 30°C
0.42
L-phenylalanine
-
pH 7.0, 25°C, with cofactor 6-methyl-tetrahydrobiopterin, recombinant mutant DELTA 117PheH V379D
0.454
L-phenylalanine
-
cofactor 6-methyl-5,6,7,8-tetrahydropterin, enzyme form II
0.4675
L-phenylalanine
mutant enzyme F258A, in 0.1 M Na-HEPES (pH 7.4) at 30°C
0.478
L-phenylalanine
mutant enzyme L101Y, at 30°C, in 50 mM HEPES-NaOH buffer (pH 7.5)
0.504
L-phenylalanine
-
cofactor 6-methyltetrahydropterin, maltose-binding-protein phenylalanine fusion protein
0.637
L-phenylalanine
-
wild type enzyme, without preincubation with L-phenylalanine, 100 mM Tris-HCl (pH 7.5), at 37°C
0.6872
L-phenylalanine
mutant enzyme S230P, in 0.1 M Na-HEPES (pH 7.4) at 30°C
0.72
L-phenylalanine
-
cofactor 6-methyl-5,6,7,8-tetrahydropterin, enzyme form I
0.87
L-phenylalanine
-
post-translational activation of PAH in human hepatic cytosol fractions, activator: NO (nitric oxide) (0.01 mM), Vmax: 274.5 nmoles/min/mg, pH 6.8, 37°C
1.1
L-phenylalanine
wild type enzyme, in 50 mM potassium phosphate buffer, pH 6.8, at 37°C
1.22
L-phenylalanine
-
post-translational activation of PAH in human hepatic cytosol fractions, activator: N-ethylmaleimide (50 mM), Vmax: 224 nmoles/min/mg, pH 6.8, 37°C
1.25
L-phenylalanine
-
post-translational activation of PAH in human hepatic cytosol fractions, activator: ONOO- (peroxynitrite)(1 mM), Vmax: 260.4 nmoles/min/mg, pH 6.8, 37°C
1.43
L-phenylalanine
-
cofactor 6,7-dimethyl-5,6,7,8-tetrahydropterin, enzyme form II
1.5 - 2
L-phenylalanine
-
post-translational activation of PAH in human hepatic cytosol fractions, activator: HO (hydroxyl radical) (0.01 mM), Vmax: 241.6 nmoles/min/mg, pH 6.8, 37°C
1.92
L-phenylalanine
-
cofactor 6,7-dimethyl-5,6,7,8-tetrahydropterin, enzyme form I
2.85
L-phenylalanine
-
post-translational activation of PAH in human hepatic cytosol fractions, activator: lysophosphatidylcholine (5 mM), Vmax: 29.1 nmoles/min/mg, pH 6.8, 37°C
3.01
L-phenylalanine
-
post-translational activation of PAH in human hepatic cytosol fractions, activator: H2O2 (0.1 mM), Vmax: 205 nmoles/min/mg, pH 6.8, 37°C
3.05
L-phenylalanine
-
post-translational activation of PAH in human hepatic cytosol fractions, activator: L-Phe (4 mM), Vmax: 14.23 nmoles/min/mg, pH 6.8, 37°C
1
L-tryptophan
mutant enzyme W180F, at 30°C, in 50 mM HEPES-NaOH buffer (pH 7.5)
3.44
L-tryptophan
wild type enzyme, at 30°C, in 50 mM HEPES-NaOH buffer (pH 7.5)
3.54
L-tryptophan
mutant enzyme L101Y, at 30°C, in 50 mM HEPES-NaOH buffer (pH 7.5)
4.06
L-tryptophan
mutant enzyme L101Y/W180F, at 30°C, in 50 mM HEPES-NaOH buffer (pH 7.5)
8.5
L-tryptophan
-
cofactor 2-amino-4-hydroxy-6,7-dimethyltetrahydropteridine, enzyme form I
55.97
N-acetyl-S-carboxymethyl-L-cysteine
-
wild type enzyme from hepatic cytosol
57.15
N-acetyl-S-carboxymethyl-L-cysteine
wild type enzyme from hepatic cytosol
63.8
N-acetyl-S-carboxymethyl-L-cysteine
wild type enzyme from Hep-G2 cell cytosol
68.25
N-acetyl-S-methyl-L-cysteine
wild type enzyme from Hep-G2 cell cytosol
0.049
phenylalanine
-
phenylalanine-activated recombinant enzyme, cofactor tetrahydrobiopterin
0.055
phenylalanine
-
recombinant enzyme, cofactor (7R)-5,6,7,8-tetrahydrobiopterin
4.6
S-carboxy-methyl-L-cysteine
-
at 37°C, 50 mM potassium phosphate buffer, pH 6.8
14.73
S-carboxy-methyl-L-cysteine
-
at 37°C, 50 mM potassium phosphate buffer, pH 6.8
0.0728
S-carboxymethyl-L-cysteine
wild type enzyme, in 50 mM potassium phosphate buffer (pH 6.8), at 37°C
0.14
S-carboxymethyl-L-cysteine
mutant enzyme R68S, in 50 mM potassium phosphate buffer (pH 6.8), at 37°C
0.143
S-carboxymethyl-L-cysteine
mutant enzyme Y414C, in 50 mM potassium phosphate buffer (pH 6.8), at 37°C
0.146
S-carboxymethyl-L-cysteine
mutant enzyme V388M, in 50 mM potassium phosphate buffer (pH 6.8), at 37°C
0.15
S-carboxymethyl-L-cysteine
mutant enzyme I65T, in 50 mM potassium phosphate buffer (pH 6.8), at 37°C
0.155
S-carboxymethyl-L-cysteine
mutant enzyme R261Q, in 50 mM potassium phosphate buffer (pH 6.8), at 37°C
2 - 3
S-carboxymethyl-L-cysteine
-
allelic combination wild-type/mutant R261Q, Vmax: 15 micromol/min/mg, pH 6.8, 37°C
2.38
S-carboxymethyl-L-cysteine
-
post-translational activation of human cDNA-expressed PAH, activator: NO (nitric oxide) (0.01 mM), Vmax: 42.32 nmoles/min/mg, pH 6.8, 37°C
2.75
S-carboxymethyl-L-cysteine
-
post-translational activation of human cDNA-expressed PAH, activator: H2O2 (0.1 mM), Vmax: 36.5 nmoles/min/mg, pH 6.8, 37°C
3.2
S-carboxymethyl-L-cysteine
-
post-translational activation of human cDNA-expressed PAH, activator: HO (hydroxyl radical) (0.01 mM), Vmax: 36.5 nmoles/min/mg, pH 6.8, 37°C
4.1
S-carboxymethyl-L-cysteine
-
post-translational activation of human cDNA-expressed PAH, activator: ONOO- (peroxynitrite)(1 mM), Vmax: 41.27 nmoles/min/mg, pH 6.8, 37°C
4.38
S-carboxymethyl-L-cysteine
-
post-translational activation of PAH in human hepatic cytosol fractions, activator: NO (nitric oxide) (0.01 mM), Vmax: 500 nmoles/min/mg, pH 6.8, 37°C
4.62
S-carboxymethyl-L-cysteine
-
post-translational activation of PAH in human hepatic cytosol fractions, activator: H2O2 (0.1 mM), Vmax: 430 nmoles/min/mg, pH 6.8, 37°C
5.23
S-carboxymethyl-L-cysteine
-
post-translational activation of PAH in human hepatic cytosol fractions, activator: N-ethylmaleimide (50 mM), Vmax: 450 nmoles/min/mg, pH 6.8, 37°C
6.49
S-carboxymethyl-L-cysteine
-
post-translational activation of PAH in human hepatic cytosol fractions, activator: HO (hydroxyl radical) (0.01 mM), Vmax: 455 nmoles/min/mg, pH 6.8, 37°C
7.54
S-carboxymethyl-L-cysteine
-
post-translational activation of PAH in human hepatic cytosol fractions, activator: ONOO- (peroxynitrite)(1 mM), Vmax: 481 nmoles/min/mg, pH 6.8, 37°C
7.65
S-carboxymethyl-L-cysteine
-
post-translational activation of human cDNA-expressed PAH, activator: N-ethylmaleimide (50 mM), Vmax: 5.58 nmoles/min/mg, pH 6.8, 37°C
8
S-carboxymethyl-L-cysteine
-
allelic combination wild-type/wild-type, Vmax: 114 micromol/min/mg, pH 6.8, 37°C
8.1
S-carboxymethyl-L-cysteine
-
post-translational activation of human cDNA-expressed PAH, activator: L-Phe (4 mM), Vmax: 0.07 nmoles/min/mg, pH 6.8, 37°C
8.3
S-carboxymethyl-L-cysteine
-
post-translational activation of human cDNA-expressed PAH, activator: lysophosphatidylcholine (5 mM), Vmax: 4.38 nmoles/min/mg, pH 6.8, 37°C
15
S-carboxymethyl-L-cysteine
-
allelic combination wild-type/mutant I65T, Vmax: 18 micromol/min/mg, pH 6.8, 37°C
16.53
S-carboxymethyl-L-cysteine
-
in pooled hepatic cytosolic enzyme fraction activated with 1 mM lysophosphatidylcholine, at 37°C
16.53
S-carboxymethyl-L-cysteine
-
post-translational activation of PAH in human hepatic cytosol fractions, activator: L-Phe (4 mM), Vmax: 0.87 nmoles/min/mg, pH 6.8, 37°C
16.53
S-carboxymethyl-L-cysteine
-
post-translational activation of PAH in human hepatic cytosol fractions, activator: lysophosphatidylcholine (5 mM), Vmax: 52.31 nmoles/min/mg, pH 6.8, 37°C
18
S-carboxymethyl-L-cysteine
-
allelic combination wild-type/mutant R68S, Vmax: 17 micromol/min/mg, pH 6.8, 37°C
19
S-carboxymethyl-L-cysteine
-
allelic combination wild-type/mutant I174T, Vmax: 2 micromol/min/mg, pH 6.8, 37°C
20
S-carboxymethyl-L-cysteine
-
allelic combination wild-type/mutant R158Q, Vmax: 3 micromol/min/mg, pH 6.8, 37°C
21
S-carboxymethyl-L-cysteine
-
allelic combination wild-type/mutant R408W, Vmax: 2 micromol/min/mg, pH 6.8, 37°C
24
S-carboxymethyl-L-cysteine
-
allelic combination wild-type/mutant Y414C, Vmax: 22 micromol/min/mg, pH 6.8, 37°C
25
S-carboxymethyl-L-cysteine
-
allelic combination wild-type/mutant V388M, Vmax: 12 micromol/min/mg, pH 6.8, 37°C
25.24
S-carboxymethyl-L-cysteine
wild type enzyme from Hep-G2 cell cytosol
0.45
S-methyl-ergothionine
-
at 37°C, 50 mM potassium phosphate buffer, pH 6.8
43.25
S-methyl-L-cysteine
-
at 37°C, 50 mM potassium phosphate buffer, pH 6.8
0.025
tetrahydrobiopterin
-
cleaved maltose-binding-protein phenylalanine fusion protein
0.03 - 0.04
tetrahydrobiopterin
-
recombinant wild-type, C237S and C237D mutant enzyme
0.031
tetrahydrobiopterin
-
maltose-binding-protein phenylalanine fusion protein
0.041
tetrahydrobiopterin
-
truncated enzyme containing C-terminal 334 amino acids
1.7
thienylalanine
-
cofactor tetrahydrobiopterin, lysolecithin activated enzyme
0.024
tryptophan
-
truncated enzyme containing C-terminal 334 amino acids, pH 8.0
0.096
tryptophan
-
truncated enzyme containing C-terminal 334 amino acids, pH 7.0
additional information
L-phenylalanine
-
wild-type, pH 7.3, 25°C, measurement by standard discontinuous PAH activity assay (HPLC and fluorimetric detection): Vmax: 0.495 micromol L-Tyr/min/mg (without L-Phe preincubation), 1.55 micromol L-Tyr/min/mg (with L-Phe preincubation), S0.5: 0.318 mM (without L-Phe preincubation), S0.5: 0.154 mM (with L-Phe preincubation)
additional information
additional information
Q9XYQ5
kinetic analysis
-
additional information
additional information
kinetics, molecular dynamics simulations, structure-energetics calculations, and molecular interaction fields, overview
-
additional information
additional information
-
thermodynamics of iron nitrosyl formation
-
additional information
additional information
thermodynamics, overview
-
additional information
additional information
-
binding constants for Fe2+ of wild-type and mutant enzymes, overview
-
additional information
additional information
-
kinetic and molecular modelling of sulfur-containing substrates, overview
-
additional information
additional information
-
kinetic and molecular modelling of sulfur-containing substrates, overview
-
additional information
additional information
-
[S]0.5 (L-phenylalanine): 0.188 mM wild-type,0,109 mM mutant Q215K/N216Y, Vmax (wild-type): 1.9 micromol L-Tyr/min/mg, Vmax (mutant Q215K/N216Y): 6.6 micromol L-Tyr/min/mg, pH 7.0, 25°C
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