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1.14.15.8: steroid 15beta-monooxygenase

This is an abbreviated version!
For detailed information about steroid 15beta-monooxygenase, go to the full flat file.

Word Map on EC 1.14.15.8

Reaction

progesterone
+
reduced ferredoxin
 +
O2
 +
H+
=
15beta-hydroxyprogesterone
 +
oxidized ferredoxin
 +
H2O

Synonyms

15beta-hydroxylase, 15beta-hydroxylase CYP106A2, BmCYP106A2, Cyp106A1, CYP106A2, cytochrome P-450meg, cytochrome P450meg, steroid-15-beta-monooxygenase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.15 With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.15.8 steroid 15beta-monooxygenase

Purification

Purification on EC 1.14.15.8 - steroid 15beta-monooxygenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
it is possible to resolve the hydroxylase system into three proteins: a strictly NADPH-dependent FMN-containing flavoprotein (megaredoxin reductase), an iron-sulfur protein (megaredoxin), and cytochrome P-450 (P-450meg). The activity of the 15beta-hydroxylase system is fully reconstituted upon combination of these three proteins and addition of NADPH. Megaredoxin has an apparent sulfur to iron ratio of 0.98 and shows g-signals at 1.90, 1.93, and 2.06 when analyzed by electron paramagnetic resonance spectroscopy
-
the purification procedure includes chromatography on DEAE-cellulose, Ultrogel ACA-54, DEAE-Sepharose, octyl-Sepharose, and hydroxyapatite