1.14.15.8: steroid 15beta-monooxygenase
This is an abbreviated version!
For detailed information about steroid 15beta-monooxygenase, go to the full flat file.
Word Map on EC 1.14.15.8
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1.14.15.8
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megaterium
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p-450-dependent
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ferredoxins
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synthesis
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adrenodoxin
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autologous
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triterpene
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11-oxidase
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testosterone
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flavodoxin
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flavoprotein
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hydroxylases
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aniline
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11-deoxycortisol
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nadph-dependent
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fmn-containing
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paramagnetic
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iron-sulfur
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biocatalyst
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11-keto
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11-deoxycorticosterone
- 1.14.15.8
- megaterium
-
p-450-dependent
- ferredoxins
- synthesis
- adrenodoxin
-
autologous
-
triterpene
-
11-oxidase
- testosterone
- flavodoxin
- flavoprotein
- hydroxylases
- aniline
- 11-deoxycortisol
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nadph-dependent
-
fmn-containing
-
paramagnetic
-
iron-sulfur
-
biocatalyst
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11-keto
- 11-deoxycorticosterone
Reaction
Synonyms
15beta-hydroxylase, 15beta-hydroxylase CYP106A2, BmCYP106A2, Cyp106A1, CYP106A2, cytochrome P-450meg, cytochrome P450meg, steroid-15-beta-monooxygenase
ECTree
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Application
Application on EC 1.14.15.8 - steroid 15beta-monooxygenase
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synthesis
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a steroid 15beta-hydroxylating whole-cell solvent tolerant biocatalyst is constructed by expressing the Bacillus megaterium steroid hydroxylase CYP106A2 in the solvent tolerant Pseudomonas putida S12. Testosterone hydroxylation is improved by a factor 16 by co-expressing Fer, a putative Fe-S protein from Bacillus subtilis. The specificity for 15beta-hydroxylation is improved by mutating threonine residue 248 of CYP106A2 into valine. These insights provide the basis for an optimized whole-cell steroid-hydroxylating biocatalyst that can be applied with an organic solvent phase
synthesis
efficient approach towards the preparative scale synthesis of hydroxylated steroid derivatives. Improve CYP106A2-catalyzed steroid hydroxylation towards higher productivity and quantitative product formation. Because substrate transport into the cell limits the whole-cell biotransformation, activity can be increased sixfold by using membrane-free crude cell as biocatalyst
synthesis
an overexpressing Bacillus megaterium strain produces up to 115 mg/l/h 7beta-hydroxydehydroepiandrosterone in whole-cell conversions