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1.14.15.7: choline monooxygenase

This is an abbreviated version!
For detailed information about choline monooxygenase, go to the full flat file.

Word Map on EC 1.14.15.7

Reaction

choline
+
O2
 +
reduced ferredoxin
 + 2 H+ =
betaine aldehyde hydrate
 +
H2O
 +
oxidized ferredoxin

Synonyms

AhCMO, AnCMO, ApCMO, choline monooxygenase, choline oxygenase, CMO, CMO1, EC 1.14.14.4, Os06g48510

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.15 With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.15.7 choline monooxygenase

Cloned

Cloned on EC 1.14.15.7 - choline monooxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
coexpression of chloroplast CMO and Spinacia oleracea betaine aldehyde dehydrogenase BADH genes in Lolium perenne via particle bombardment
-
DNA and amino acid sequence determination and analysis, genetic structure, most rice CMO transcripts are processed incorrectly, retaining introns or deleted of coding sequences, the unusual deletion events occurr at sequence elements of the short-direct repeats
expression analysis at normal and abiotic stress conditions, overview
-
expression in Escherichia coli
expression in Escherichia coli, Synechococcus sp. PCC7942 and Arabidopsis thaliana
expression of CMO via plastid genetic engineering in transgenic Nicotiana tabacum cv. Wisconsin 38 plants, which show accumulation of glycine betaine and increased tolerance to abiotic stress
-
gene CMO, DNA and amino acid sequence determination and analysis, phylogenetic tree, recombinant expression in transgenic Nicotiana tabacum plants via Agrobacterium tumefaciens strain LBA4404 transfection system leading to increased salt tolerance in the transgenic plants
gene HvCMO, DNA and amino acid sequence determination and analysis, phylogenetic tree, quantitative real-time PCR expression analyis, recombinant expression in Saccharomyces cerevisiae strain BJ5458
gene OsCMO, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional OsCMO expression in transgenic Nicotiana tabacum cv. Xanthinc plants using Agrobacterium tumefaciens EHA105 for transfection, overexpressing plants show increased glycine betaine content and elevated tolerance to salt stress
Gossypium hirsutum is transformed with the Atriplex hortensis CMO gene mediated by Agrobacterium tumefaciens
into a TA cloning system for sequencing
overexpression in Oryza sativa by Agrobacterium tumefaciens-mediated transformation, single copy of the transgene
-
quantitative real-time reverse transcriptase PCR expression analysis
-