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1.14.15.3: alkane 1-monooxygenase

This is an abbreviated version!
For detailed information about alkane 1-monooxygenase, go to the full flat file.

Word Map on EC 1.14.15.3

Reaction

Octane
+
reduced rubredoxin
+
O2
+ 2 H+ =
1-Octanol
+
oxidized rubredoxin
+
H2O

Synonyms

1-hydroxylase, alkane 1-hydrolase, alkane hydroxylase, alkane mono-oxygenase, alkane monooxygenase, alkane omega-hydroxylase, alkane-1-monooxygenase 1, alkane-1-monooxygenase 2, AlkB, AlkB1, AlkB2, AlkW1, AlkW2, AlmA, AlmA-like alkane hydroxylase, BMO, butane monooxygenase, CYP omega hydroxylase, CYP omega-hydroxylase, Cyp153, CYP153 homologue, CYP153A, CYP153D17, CYP4 P450, CYP450 4A omega-hydroxylase, CYP450 omega-hydroxylase, CYP4A, CYP4A10, CYP4A11, CYP4A14, CYP4AII, CYP4d21, CYP86A1, CYP86B1, CYP94A1, CYPA11 P450, CYPIVA1, CYPIVA11, CYPIVA2, CYPIVA3, CYPIVA5, CYPIVA6, CYPIVA7, cytochrome P-450 omega-hydroxylase, cytochrome P450 alkane hydroxylase, cytochrome P450 omega hydroxylase, cytochrome P450-dependent fatty acid omega-hydroxylase, diiron omega-hydroxylase, FA omega-hydroxylase, fatty acid omega-hydrolase, Fatty acid omega-hydroxylase, Gpo1, hexane hydroxylase, Lauric acid omega-hydroxylase, MAH1-1, MAH1-2, male-specific fatty acid omega-hydroxylase, medium-chain alkane hydroxylase, methane monooxygenase, More, omega hydroxylase, omega-hydrolase, omega-hydroxylase, P-450 HK omega, P450-HL-omega, P452, Prm1ABCD, Prm2ABCD, propane monooxygenase, Rieske-type alkane monooxygenase, sex-specific enzyme 1, SXE1, UAM-Ps1, very long chain fatty acid hydroxylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.15 With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.15.3 alkane 1-monooxygenase

Cloned

Cloned on EC 1.14.15.3 - alkane 1-monooxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
alkB genes expressed in Escherichia coli und Pseudomonas fluorescence
all components of the alkane ydroxylase system expressed in Escherichia coli TOP10
-
baculovirus expressed human CYP4A11
-
CYP86B1-YFP fusion protein expressed under the control of the 35S promoter in a transient expression assay in Nicotiana benthamiana leaves. ProCYP86B1::GUS transgenic plants
expressed in Escherichia coli , human gene CYP4AII
-
expressed in Escherichia coli BL21(DE3)pLysS
-
expressed in Escherichia coli strain XL1 and SCS1
-
expressed in Escherichia coli XL-1 blue, human gene CYP4A1
-
expressed in Escherichia coli, genes CYP4A1, CYP4A2, CYP4A3 and CYP4A8
-
expressed in Escherichia coli, human gene CYP4A11
-
expressed in Pseudomonas fluorescence KOB2delta1
expressed in Pseudomonas putida strain GPo12
expressed in Saccharomyces cerevisiae strain GRF18
-
expressed in Saccharomyces cerevisiae strain WAT11
-
expression in Escherichia coli
gene alkB, expression of C-terminally StrepII-tagged AlkB in membranes of Escherichia coli strain NovaBlue
gene alkB, expression of Strep-tagged enzyme in Escherichia coli strains BL21(DE3), C41(DE3), Rosetta-2(DE3), pLysS, and BL21-CondonPlus(DE3)-RIPL under different growth conditions
-
gene alkW1, DNA and amino acid sequence determination and analysis, genetic organization, sequence comparisons and phylogenetic analysis, quantitative RT-PCR expression analysis, heterologous expression of alkW1 in alkB-deficient P.seudomonas fluorescens and functional complementation
gene alkW2, DNA and amino acid sequence determination and analysis, genetic organization, sequence comparisons and phylogenetic analysis, quantitative RT-PCR expression analysis
genes alkB1, alkB2, p450 and almA, DNA and amino acid sequence determination and analysis, genetic organization, sequence comparisons and phylogenetic analysis, one copy of alkB1 in the B-5 chromosome. Expression of genes alkB1, alkB2, p450 and almA in Pseudomonas putida GPo12 pGEc47DELTAB or Pseudomonas fluorescens KOB2DELTA1, quantitative real-time RT-PCR expression analysis
-
recombinant expression of His6-tagged enzyme system components in Escherichia coli strain BL21(DE3)
-
the 1.4 kb putative promoter region upstream of the predicted CYP86A1 start codon fused in frame to the beta-glucuronidase gene and used to transform wild-type Arabidopsis under the control of the native promoter. Transgenic expression of CYP86A1 fused to GFP, CYP86A1:GFP heterologously and transiently expressed under the control of the CaMV35S-promoter in the epidermis of Nicotiana benthamiana leaves