1.14.15.22: vitamin D 1,25-hydroxylase

This is an abbreviated version!
For detailed information about vitamin D 1,25-hydroxylase, go to the full flat file.

Word Map on EC 1.14.15.22

Reaction

calcidiol
+ 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ +
O2
=
calcitriol
+ 2 oxidized ferredoxin [iron-sulfur] cluster +
H2O

Synonyms

CYP105A1, cytochrome P450SU-1, Streptomyces griseolus cytochrome P450SU-1, vitamin D3 dihydroxylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.15 With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.15.22 vitamin D 1,25-hydroxylase

Crystallization

Crystallization on EC 1.14.15.22 - vitamin D 1,25-hydroxylase

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CRYSTALLIZATION/commentary
ORGANISM
UNIPROT
LITERATURE
structure of the mutant R73V/R84A with 1alpha,25-dihydroxyvitamin D3, a hydrogen-bond network including the 1alpha-hydroxyl group and several water molecules play an important role in the substrate-binding for 26-hydroxylation
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wild-type to 1.5 A resolution, and mutant R84A, native protein and in complex with 1alpha,25-dihydroxyvitamin D3. The compound is positioned 11 A from the iron atom along the I helix within the pocket. The loss of two hydrogen bonds in the R84A mutant increases the adaptability of the B and F helices, creating a transient binding site
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