Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.14.15.16: vitamin D3 24-hydroxylase

This is an abbreviated version!
For detailed information about vitamin D3 24-hydroxylase, go to the full flat file.

Word Map on EC 1.14.15.16

Reaction

calcidiol
+ 2 reduced adrenodoxin + 2 H+ +
O2
=
secalciferol
 + 2 oxidized adrenodoxin +
H2O

Synonyms

1,25-(OH)2D3-24-hydroxylase, 1,25-dihydroxyvitamin D3 24-hydroxylase, 1alpha,25-dihydroxyvitamin D3 24-hydroxylase, 24-hydroxylase, 24-OHase, 24OHase, 25-hydroxyvitamin D-24-hydroxylase, 25-hydroxyvitamin D3-24-hydroxylase, 25-OH-D3-24-hydroxylase, CYP24A1, cytochrome P450 24A1, EC 1.14.13.126, P450cc24, vitamin D 24-hydroxylase, vitamin D-24-hydroxylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.15 With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.15.16 vitamin D3 24-hydroxylase

Engineering

Engineering on EC 1.14.15.16 - vitamin D3 24-hydroxylase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A326G
E143del
naturally occuring mutation
E147del
naturally occuring mutation
E149del
naturally occuring mutation
E151X
naturally occuring mutation
E322K
naturally occuring mutation
L409S
naturally occuring missense mutation, found on only one allele, no other mutation is found in exons or in at least 30 bp of each intron/exon junction. ThecL409S mutant has about 32% of wild-type 24-hydroxylase activity
R159Q
naturally occuring mutation
R396W
naturally occuring mutation
V391L
-
the mutation converts the enzyme from a catabolic 1alpha,25-dihydroxyvitamin-D3-24-hydroxylase into an anabolic 1alpha-hydroxyvitamin-D3-25-hydroxylase. The mutant enzyme retains its basal ability to catabolize 1alpha,25-dihydroxyvitamin D3 via C24 hydroxylation
V391L/A326G
-
the mutant enzyme continues to form 1alpha,25-dihydroxyvitamin D3 from 1alpha-hydroxyvitamin-D3, but this initial product is diverted via the C23 hydroxylation pathway into 1alpha,25-dihydroxyvitamin-D3-26,23-lactone
W210R
naturally occuring mutation
W268X
naturally occuring mutation
I500F
-
the mutant shows quite a different metabolism of 1alpha,25-dihydroxyvitamin D3 from both human and rat CYP24A1
I500L
-
the mutant also shows the C-23 oxidation pathway
I500T
-
the mutant also shows the C-23 oxidation pathway
I500V
-
the mutant also shows the C-23 oxidation pathway
S57D
-
site-directed mutagenesis
T416A
-
the metabolic pattern by the mutant remains rat CYP24A1wild type
T416F
-
the mutant has reaction specificity similar to human CYP24A1 by also showing the C-23 oxidation pathway
T416I
-
the mutant has reaction specificity similar to human CYP24A1 by also showing the C-23 oxidation pathway
T416M
-
the mutant also shows the C-23 oxidation pathway
T416S
-
the metabolic pattern by the mutant remains rat CYP24A1wild type
T416V
-
the mutant has reaction specificity similar to human CYP24A1 by also showing the C-23 oxidation pathway
additional information