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1.14.15.16: vitamin D3 24-hydroxylase

This is an abbreviated version!
For detailed information about vitamin D3 24-hydroxylase, go to the full flat file.

Word Map on EC 1.14.15.16

Reaction

calcidiol
+ 2 reduced adrenodoxin + 2 H+ +
O2
=
secalciferol
+ 2 oxidized adrenodoxin +
H2O

Synonyms

1,25-(OH)2D3-24-hydroxylase, 1,25-dihydroxyvitamin D3 24-hydroxylase, 1alpha,25-dihydroxyvitamin D3 24-hydroxylase, 24-hydroxylase, 24-OHase, 24OHase, 25-hydroxyvitamin D-24-hydroxylase, 25-hydroxyvitamin D3-24-hydroxylase, 25-OH-D3-24-hydroxylase, CYP24A1, cytochrome P450 24A1, EC 1.14.13.126, P450cc24, vitamin D 24-hydroxylase, vitamin D-24-hydroxylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.15 With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.15.16 vitamin D3 24-hydroxylase

Cloned

Cloned on EC 1.14.15.16 - vitamin D3 24-hydroxylase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in chinese hamster lung fibroblast cells (V79-4 cells)
-
expressed in Escherichia coli
expressed in Escherichia coli BL21-Gold(DE3) and Rosetta 2(DE3) cells
-
expressed in Escherichia coli JM109 cells
expressed in Sf21 insect cells using the previously cloned cDNA in baculovirus (AcNPV-P450cc24), solubilized mitochondrial protein from Sf21 cells infected with recombinant virus AcNPV-P450cc24 cDNA express 24-hydroxylase activity when reconstituted with bovine adrenodoxin, adrenodoxin reductase, and NADPH
-
expressed in V79-CYP24 or OK (CCL-1840) cells
-
gene CYP24A1, cloned from leukocyte DNA, DNA and amino acid sequence determination and analysis, expression analysis by RT-PCR, recombinant expression of wild-type and mutant CYP24A1 cDNAs in JEG-3 cells. The mammalian two-hybrid assay system consisting of the retinoid-X receptor and vitamin D receptor in JEG-3 cells is used as a simple, sensitive, quantitative assay of enzyme activity
gene CYP24A1, functional recombinant His-tagged enzyme expression in Escherichia coli membranes
gene CYP24A1, overexpression of N-terminally maltose-binding-protein fused enzyme in Escherichia coli
gene CYP24A1, recombinant expression of N-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain DH5alpha F'IQ
-
mutant enzymes are expressed in Escherichia coli BL21(DE3) cells
-