1.14.15.16: vitamin D3 24-hydroxylase
This is an abbreviated version!
For detailed information about vitamin D3 24-hydroxylase, go to the full flat file.
Word Map on EC 1.14.15.16
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1.14.15.16
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cyp24a1
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parathyroid
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1alpha-hydroxylase
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rickets
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24,25-dihydroxyvitamin
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hypercalcemia
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24-hydroxylation
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retinoids
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d-dependent
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1alpha,25oh2d3
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osteocalcin
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vdres
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d-deficient
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1-hydroxylase
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d-responsive
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1,25-dihydroxycholecalciferol
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hyperparathyroidism
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ketoconazole
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nephrocalcinosis
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alopecia
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vdr-mediated
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hypophosphatemic
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d-binding
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1,25oh2d3-induced
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extrarenal
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hypercalciuria
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cholecalciferol
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25-hydroxycholecalciferol
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calcemic
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1alpha-ohase
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alpha-hydroxyvitamin
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calcidiol
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d-resistant
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d3-1alpha-hydroxylase
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osteomalacia
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d-replete
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secosteroid
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hvdrr
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sodium-phosphate
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medicine
- 1.14.15.16
- cyp24a1
- parathyroid
-
1alpha-hydroxylase
- rickets
-
24,25-dihydroxyvitamin
- hypercalcemia
-
24-hydroxylation
-
retinoids
-
d-dependent
-
1alpha,25oh2d3
- osteocalcin
-
vdres
-
d-deficient
- 1-hydroxylase
-
d-responsive
- 1,25-dihydroxycholecalciferol
- hyperparathyroidism
- ketoconazole
- nephrocalcinosis
- alopecia
-
vdr-mediated
-
hypophosphatemic
-
d-binding
-
1,25oh2d3-induced
-
extrarenal
-
hypercalciuria
- cholecalciferol
- 25-hydroxycholecalciferol
-
calcemic
- 1alpha-ohase
-
alpha-hydroxyvitamin
- calcidiol
-
d-resistant
-
d3-1alpha-hydroxylase
-
osteomalacia
-
d-replete
-
secosteroid
-
hvdrr
-
sodium-phosphate
- medicine
Reaction
+ 2 reduced adrenodoxin + 2 H+ + = + 2 oxidized adrenodoxin +
Synonyms
1,25-(OH)2D3-24-hydroxylase, 1,25-dihydroxyvitamin D3 24-hydroxylase, 1alpha,25-dihydroxyvitamin D3 24-hydroxylase, 24-hydroxylase, 24-OHase, 24OHase, 25-hydroxyvitamin D-24-hydroxylase, 25-hydroxyvitamin D3-24-hydroxylase, 25-OH-D3-24-hydroxylase, CYP24A1, cytochrome P450 24A1, EC 1.14.13.126, P450cc24, vitamin D 24-hydroxylase, vitamin D-24-hydroxylase
ECTree
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Cloned
Cloned on EC 1.14.15.16 - vitamin D3 24-hydroxylase
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expressed in Escherichia coli
expressed in Escherichia coli JM109 cells
expressed in Sf21 insect cells using the previously cloned cDNA in baculovirus (AcNPV-P450cc24), solubilized mitochondrial protein from Sf21 cells infected with recombinant virus AcNPV-P450cc24 cDNA express 24-hydroxylase activity when reconstituted with bovine adrenodoxin, adrenodoxin reductase, and NADPH
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gene CYP24A1, cloned from leukocyte DNA, DNA and amino acid sequence determination and analysis, expression analysis by RT-PCR, recombinant expression of wild-type and mutant CYP24A1 cDNAs in JEG-3 cells. The mammalian two-hybrid assay system consisting of the retinoid-X receptor and vitamin D receptor in JEG-3 cells is used as a simple, sensitive, quantitative assay of enzyme activity
gene CYP24A1, functional recombinant His-tagged enzyme expression in Escherichia coli membranes
gene CYP24A1, overexpression of N-terminally maltose-binding-protein fused enzyme in Escherichia coli
gene CYP24A1, recombinant expression of N-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain DH5alpha F'IQ
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