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1.14.15.1: camphor 5-monooxygenase

This is an abbreviated version!
For detailed information about camphor 5-monooxygenase, go to the full flat file.

Word Map on EC 1.14.15.1

Reaction

(+)-Camphor
+
reduced putidaredoxin
+
O2
=
(+)-exo-5-hydroxycamphor
+
oxidized putidaredoxin
+
H2O

Synonyms

2-bornanone 5-exo-hydroxylase, bornanone 5-exo-hydroxylase, CamC, camphor 5-exo-hydroxylase, camphor 5-exo-methylene hydroxylase, camphor 5-exohydroxylase, camphor 5-hydroxylase, Camphor 5-monooxygenase, camphor hydroxylase, camphor hydroxylase cytochrome P450cam, camphor methylene hydroxylase, camphor monooxygenase, class I cytochrome P450, CYP101, CYP101A1, CYP101B1, CYP101C1, CYP101D1, CYP101D2, CYP111A2, Cyt P450cam, cytochrome P-450-CAM, cytochrome P450 cam, cytochrome P450(cam), cytochrome p450cam, cytochrome P450cam monooxygenase, d-camphor monooxygenase, D-camphor-exo-hydroxylase, haem mono-oxygenase CYP101, methylene hydroxylase, methylene monooxygenase, moe, oxygenase, camphor 5-mono-, P450cam, P450cam monooxygenase, P450tcu

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.15 With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.15.1 camphor 5-monooxygenase

Purification

Purification on EC 1.14.15.1 - camphor 5-monooxygenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by anion exchange, hydrophobic interaction and gel filtration
-
by gel filtration
-
native C334A enzyme mutant from Pseudomonas putida strain ATCC 17453 by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration
-
recombinant C334A enzyme mutant from Escherichia coli strain NCM533 by ammonium sulfate fractionation, dialysis, anion exchange chromatography, and gel filtration
-
recombinant enzyme from Escherichia coli strain BL21(DE3)
recombinant enzyme to 90% purity from Escherichia coli strain DH5alpha
-
recombinant His-tagged CYP101D2 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and anion exchange chromatography to over 95% purity
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain XL-1 Blue by nickel affinity chromatography and repeated anion exchange chromatography
recombinant N--terminally His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)
recombinant P450cam C334A from Escherichia coli NCM533. Protein purification includes a protamine sulfate cut to precipitate nucleic acids, and an ammonium sulfate cut to isolate CYP101A1
recombinant P450cam mutant C334A from Escherichia coli strain BL21(DE3) by anion exchange chromatography and gel filtration
recombinant wild-type and mutant enzymes from Escherichia coli by anion exchange chromatography and gel filtration to homogeneity
recombinant wild-type and mutant enzymes from Escherichia coli by anion exchange chromatography and hydrophobic interaction chromatography
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by a process including anion exchange chromatography and gel filtration
recombinant wild-type enzyme and chimeric enzyme fusion protein P450cam-PdR from Escherichia coli strain BL21 (DE3)
soluble proteins separated by ultracentrifugation and purified using Ni2+-nitrilotriacetate column and gel filtration
to homogeneity by SDS–PAGE
wild-type and mutants