1.14.15.1: camphor 5-monooxygenase

This is an abbreviated version!
For detailed information about camphor 5-monooxygenase, go to the full flat file.

Reaction

Synonyms

2-bornanone 5-exo-hydroxylase, bornanone 5-exo-hydroxylase, CamC, camphor 5-exo-hydroxylase, camphor 5-exo-methylene hydroxylase, camphor 5-exohydroxylase, camphor 5-hydroxylase, Camphor 5-monooxygenase, camphor hydroxylase, camphor hydroxylase cytochrome P450cam, camphor methylene hydroxylase, camphor monooxygenase, class I cytochrome P450, CYP101, CYP101A1, CYP101B1, CYP101C1, CYP101D1, CYP101D2, CYP111A2, Cyt P450cam, cytochrome P-450-CAM, cytochrome P450 cam, cytochrome P450(cam), cytochrome p450cam, cytochrome P450cam monooxygenase, d-camphor monooxygenase, D-camphor-exo-hydroxylase, haem mono-oxygenase CYP101, methylene hydroxylase, methylene monooxygenase, moe, oxygenase, camphor 5-mono-, P450cam, P450cam monooxygenase, P450tcu

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.15 With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor

                1.14.15.1 camphor 5-monooxygenase

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Results	in table
6	Activating Compound
78	AA Sequence
8	Application
1	CAS Registry Number
33	Cloned(Commentary)
126	Cofactor
22	Crystallization (Commentary)
21	General Information
7	General Stability
5	Inhibitors
7	kcat/KM [mM/s]
2	Ki Value [mM]
38	KM Value [mM]
16	Localization
48	Metals/Ions
8	Molecular Weight [Da]
62	Natural Substrates/ Products (Substrates)
1	Organic Solvent Stability
144	Organism
1	Oxidation Stability
4	Pathway
158	PDB ID
33	pH Optimum
2	pH Range
2	Posttranslational Modification
159	Protein Variants
26	Purification (Commentary)
21	Reaction
3	Reaction Type
105	Reference
3	Source Tissue
3	Specific Activity [micromol/min/mg]
7	Storage Stability
208	Substrates and Products (Substrate)
15	Subunits
135	Synonyms
1	Systematic Name
26	Temperature Optimum [°C]
3	Temperature Range [°C]
1	Temperature Stability [°C]
33	Turnover Number [1/s]

Activating Compound

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Activating Compound on EC 1.14.15.1 - camphor 5-monooxygenase

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ACTIVATING COMPOUND

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

bis(2-ethylhexyl) sulfosuccinate

Pseudomonas putida

-

improves the initial activity of P450cam in two-phase emulsions with initial camphor concentrations of 5-15 mM. P450cam is activated in the surfactant-free emulsions, and addition of bis(2-ethylhexyl) sulfosuccinate sodium salt improves the activity even further, at least over the range of camphor concentrations for which initial rates are readily measurable in all media. The largest rate enhancement is 4.5fold. Nearly 50times more product is formed in the surfactant-stabilized emulsions than is achieved in aqueous buffer

702799

cumene hydroperoxide

Pseudomonas putida

-

considerable amount of the putative Cpd II species accumulates, even at pH 7.4. By contrast, reactions with meta-chloroperbenzoic acid at pH 7.4 yield very little of the ca. 420 nm species, unless methanol is included

704713

glycerol

Pseudomonas putida

-

activates in vivo and in vitro

671535

methanol

Pseudomonas putida

-

reaction with meta-chloroperbenzoic acid in the presence of methanol (3% or ca. 1 M after mixing) at pH 7.4 and 25°C results in the accumulation of a considerable fraction of the P450cam as the putative Cpd II species

704713

tetrahydrofuran

Pseudomonas sp.

-

activation

347690

additional information

Pseudomonas putida

-

involvement of X-proline isomerization in enzyme function

706783

-