1.14.14.B11: nicotine demethylase
This is an abbreviated version!
For detailed information about nicotine demethylase, go to the full flat file.
Word Map on EC 1.14.14.B11
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1.14.14.B11
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nornicotine
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nicotiana
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tabacum
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n-demethylation
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n'-nitrosonornicotine
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tobacco-specific
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nitrosamine
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burley
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demethylases
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tomentosiformis
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curing
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sylvestris
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s-nicotine
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nonconverter
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nutrition
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rnai-induced
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elicitor-induced
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agriculture
- 1.14.14.B11
- nornicotine
- nicotiana
- tabacum
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n-demethylation
- n'-nitrosonornicotine
-
tobacco-specific
-
nitrosamine
-
burley
- demethylases
- tomentosiformis
-
curing
- sylvestris
-
s-nicotine
-
nonconverter
- nutrition
-
rnai-induced
-
elicitor-induced
- agriculture
Reaction
Synonyms
CYP82E, CYP82E1, CYP82E10, CYP82E2, CYP82E21, CYP82E3, CYP82E4, CYP82E4v1, CYP82E5v2, nicotine N-demethylase, NND
ECTree
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Engineering
Engineering on EC 1.14.14.B11 - nicotine demethylase
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C330W
K269N/I274Null
isoform CYP82E3 variant, no catalytic activity
K269N/I274Null/T279A
isoform CYP82E3 variant, no catalytic activity
additional information
analysis of polymorphic variations of the CYP82E2 orthologs in Nicotiana sylvestris and various Nicotiana tabacum genotypes
additional information
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analysis of polymorphic variations of the CYP82E2 orthologs in Nicotiana sylvestris and various Nicotiana tabacum genotypes
additional information
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inhibition of gene expression by RNAi leads to up ot sixfold decrease in nornicotine content with concomitant decrease in N'-nitrosonornicotine and total tobacco-specific nitrosamines
additional information
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while the nornicotine content of most commercial burley tobacco is low, a process termed conversion can bestow considerably increased nornicotine levels in a portion of the plants within the population. Transcript accumulation of isoform CYP82E4 is enhanced as much as 80fold in converter vs nonconverter tobacco. An optimized RNAi construct 82E4Ri298 suppresses nicotine to nornicotine conversion from 98% to as low as 0.8% in a strong converter tobacco line, a rate of nornicotine production that is about 3.6fold lower than typically detected in commercial varieties. Greenhouse-grown transgenic plants transformed with the RNAi construct are morphologically indistinguishable from the empty vector or wild-type controls
additional information
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wild-type isoform CYP82E3 shares 95% predicted amino acid sequence identity with isoform CYP82E4 but conferrs no activity when expressed in yeast or tobacco. Series of domain-swapping experiments between isoforms CYP82E3 and CYP82E4 to isentify regions and amino acids required for catalytic activity
additional information
Q38Q86
enzyme silencing by CYP82E21-specific RNAi expression. CYP82E4v1-interference affects the accumulation of nicotine and nornicotine, and the RNAi vector for CYP82E4v1 effectively silences CYP82E4v1 expression and further blocks the synthesis of nornicotine
additional information
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enzyme silencing by CYP82E21-specific RNAi expression. CYP82E4v1-interference affects the accumulation of nicotine and nornicotine, and the RNAi vector for CYP82E4v1 effectively silences CYP82E4v1 expression and further blocks the synthesis of nornicotine
additional information
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enzyme silencing by introducing a CYP82E21-specific RNAi construct in ovaries, silencing of CYP82E21 leads to up to 90% reduced nicotine conversion in flower ovaries
additional information
wild-type isoform CYP82E3 shares 95% predicted amino acid sequence identity with isoform CYP82E4 but confers no activity when expressed in yeast or tobacco. Series of domain-swapping experiments between isoforms CYP82E3 and CYP82E4 to identify regions and amino acids required for catalytic activity
additional information
wild-type isoform CYP82E3 shares 95% predicted amino acid sequence identity with isoform CYP82E4 but confers no activity when expressed in yeast or tobacco. Series of domain-swapping experiments between isoforms CYP82E3 and CYP82E4 to identify regions and amino acids required for catalytic activity
additional information
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wild-type isoform CYP82E3 shares 95% predicted amino acid sequence identity with isoform CYP82E4 but confers no activity when expressed in yeast or tobacco. Series of domain-swapping experiments between isoforms CYP82E3 and CYP82E4 to identify regions and amino acids required for catalytic activity
additional information
wild-type isoform CYP82E3 shares 95% predicted amino acid sequence identity with isoform CYP82E4 but confers no activity when expressed in yeast or tobacco. Series of domain-swapping experiments between isoforms CYP82E3 and CYP82E4 to isentify regions and amino acids required for catalytic activity
additional information
wild-type isoform CYP82E3 shares 95% predicted amino acid sequence identity with isoform CYP82E4 but confers no activity when expressed in yeast or tobacco. Series of domain-swapping experiments between isoforms CYP82E3 and CYP82E4 to isentify regions and amino acids required for catalytic activity
additional information
-
wild-type isoform CYP82E3 shares 95% predicted amino acid sequence identity with isoform CYP82E4 but confers no activity when expressed in yeast or tobacco. Series of domain-swapping experiments between isoforms CYP82E3 and CYP82E4 to isentify regions and amino acids required for catalytic activity