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1.14.14.B11: nicotine demethylase

This is an abbreviated version!
For detailed information about nicotine demethylase, go to the full flat file.

Word Map on EC 1.14.14.B11

Reaction

(R)-nicotine
+
O2
+
[reduced NADPH-hemoprotein reductase]
+
O2
=
(R)-nornicotine
+
formaldehyde
+
[oxidized NADPH-hemoprotein reductase]
+
H2O

Synonyms

CYP82E, CYP82E1, CYP82E10, CYP82E2, CYP82E21, CYP82E3, CYP82E4, CYP82E4v1, CYP82E5v2, nicotine N-demethylase, NND

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.B11 nicotine demethylase

Cloned

Cloned on EC 1.14.14.B11 - nicotine demethylase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
D121-AA8 cDNA transformed into yeast cells
expression in Escherichia coli
-
expression in Saccharomyces cerevisiae
expression in yeast
gene CYP82E21, unrooted phylogenetic analysis and tree of CYP82E21 and related CYP82E coding sequences, quantitative PCR enzyme expression analysis, ectopic expression of the coding sequence in a tobacco line lacking functional CYP82E4, CYP82E5 and CYP82E10 genes, resulting in an eightfold increase of nicotine demethylation compared to the control plants confirming the nicotine N-demethylase activity of CYP82E21
-
gene CYP82E4v1, DNA and amino acid sequence determination and analysis, real-time PCR enzyme expression analysis
Q38Q86