1.14.14.9: 4-hydroxyphenylacetate 3-monooxygenase
This is an abbreviated version!
For detailed information about 4-hydroxyphenylacetate 3-monooxygenase, go to the full flat file.
Word Map on EC 1.14.14.9
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1.14.14.9
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flavin
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3,4-dihydroxyphenylacetate
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baumannii
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flavin-dependent
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tyrosol
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hydroxytyrosol
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fmnh
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two-protein
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piceatannol
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synthesis
- 1.14.14.9
- flavin
- 3,4-dihydroxyphenylacetate
- baumannii
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flavin-dependent
- tyrosol
- hydroxytyrosol
- fmnh
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two-protein
- piceatannol
- synthesis
Reaction
Synonyms
4 HPA 3-hydroxyylase, 4-HPA hydroxylase, 4-hydroxyphenylacetate 3-hydroxylase, 4-hydroxyphenylacetic acid 3-hydroxylase, 4HPA 3-monooxygenase, 4HPA3H, C2-hpah, EC 1.14.13.3, HPA 3-hydroxylase, HpaB, hpaBC, HpaC, HPAH, More, p-hydroxyphenylacetate 3-hydroxylase, p-hydroxyphenylacetate hydroxylase, p-hydroxyphenylacetic 3-hydroxylase, TPY_2462, two-component p-hydroxyphenylacetate hydroxylase
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Reaction
Reaction on EC 1.14.14.9 - 4-hydroxyphenylacetate 3-monooxygenase
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4-hydroxyphenylacetate + FADH2 + O2 = 3,4-dihydroxyphenylacetate + FAD + H2O
two-protein enzyme: the flavoprotein catalyzes the non-productive oxidation of NADH without substrate hydroxylation and the coupling protein is an absolute requirement for productive hydroxylation. Reaction mechanism and formation of 3 flavin-oxygen intermediates are studied
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4-hydroxyphenylacetate + FADH2 + O2 = 3,4-dihydroxyphenylacetate + FAD + H2O
member of novel two-component flavin-diffusible monooxygenase family, both components required for hydroxylation. The genes are located on the same operon. Physical interaction between HpaB and HpaC is not required, enhancement of activity by direct interaction is not excluded. Contains reductase component
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4-hydroxyphenylacetate + FADH2 + O2 = 3,4-dihydroxyphenylacetate + FAD + H2O
different from other hydroxylases. Two-protein component enzyme: component C1 is a flavoprotein and C2 the hydroxylase component. Mechanism: 4-hydroxyphenylacetate binds to C1, then the enzyme-bound flavin is reduced by NADH, this reduced flavin is transferred to C2 and the reoxidation of the flavin occurs concurrently with the hydroxylation of the substrate
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4-hydroxyphenylacetate + FADH2 + O2 = 3,4-dihydroxyphenylacetate + FAD + H2O
genes encoding the first four enzymes of the 4-hydroxyphenylacetic acid meta-pathway exist as two operons, one containing the hydroxylase gene while the other contains the other three
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4-hydroxyphenylacetate + FADH2 + O2 = 3,4-dihydroxyphenylacetate + FAD + H2O
family of external flavoprotein monooxygenases. The activity is induced by the substrates
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4-hydroxyphenylacetate + FADH2 + O2 = 3,4-dihydroxyphenylacetate + FAD + H2O
dehydration of the C4a-hydroxyflavin is the rate-determining step in catalysis
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4-hydroxyphenylacetate + FADH2 + O2 = 3,4-dihydroxyphenylacetate + FAD + H2O
new type of FADH-utilizing monooxygenase
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4-hydroxyphenylacetate + FADH2 + O2 = 3,4-dihydroxyphenylacetate + FAD + H2O
two protein component enzyme: flavin-containing hydroxylase and coupling protein that appears to be an effector protein, both needed for productive hydroxylation. Enzyme synthesis is induced by 3- or 4-hydroxyphenylacetate
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4-hydroxyphenylacetate + FADH2 + O2 = 3,4-dihydroxyphenylacetate + FAD + H2O
two enzymes suggested to be required for hydroxylase activity, member of new family of two-component aromatic hydroxylases. The hydroxylase, a flavoprotein, is encoded by hpaB and the coupling protein by hpaC gene
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4-hydroxyphenylacetate + FADH2 + O2 = 3,4-dihydroxyphenylacetate + FAD + H2O
enzymes of 4-hydroxyphenylacetic acid meta-cleavage pathway are sythesized following at least two sequential inductive events
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4-hydroxyphenylacetate + FADH2 + O2 = 3,4-dihydroxyphenylacetate + FAD + H2O
enzymes of 4-hydroxyphenylacetic acid meta-cleavage pathway are sythesized following at least two sequential inductive events
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4-hydroxyphenylacetate + FADH2 + O2 = 3,4-dihydroxyphenylacetate + FAD + H2O
kinetic reaction mechanisms of the smaller FMN reductase enzyme component C1 and the larger oxygenase enzyme component C2, C2 shows a random order kinetic mechanism, detailed overview
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4-hydroxyphenylacetate + FADH2 + O2 = 3,4-dihydroxyphenylacetate + FAD + H2O
kinetics and reaction mechanism
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4-hydroxyphenylacetate + FADH2 + O2 = 3,4-dihydroxyphenylacetate + FAD + H2O
catalytic mechanism and structure-function relationship, overview
4-hydroxyphenylacetate + FADH2 + O2 = 3,4-dihydroxyphenylacetate + FAD + H2O
kinetic mechanism of enzyme components C1 and C2
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4-hydroxyphenylacetate + FADH2 + O2 = 3,4-dihydroxyphenylacetate + FAD + H2O
structural basis of the catalytic mechanism of HpaB
4-hydroxyphenylacetate + FADH2 + O2 = 3,4-dihydroxyphenylacetate + FAD + H2O
kinetic reaction mechanism, overview
4-hydroxyphenylacetate + FADH2 + O2 = 3,4-dihydroxyphenylacetate + FAD + H2O
enzymes of 4-hydroxyphenylacetic acid meta-cleavage pathway are sythesized following at least two sequential inductive events
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4-hydroxyphenylacetate + FADH2 + O2 = 3,4-dihydroxyphenylacetate + FAD + H2O
structural basis of the catalytic mechanism of HpaB
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
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