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1.14.14.60: ferruginol monooxygenase

This is an abbreviated version!
For detailed information about ferruginol monooxygenase, go to the full flat file.

Reaction

ferruginol
+
[reduced NADPH-hemoprotein reductase]
+
O2
=
11-hydroxyferruginol
+
[oxidized NADPH-hemoprotein reductase]
+
H2O

Synonyms

Cyp76AH22, Cyp76AH23, CYP76AH24, CYP76AH3

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.60 ferruginol monooxygenase

Engineering

Engineering on EC 1.14.14.60 - ferruginol monooxygenase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
F112L
-
mutant catalyzes ferruginol production in yeast 2 times more efficiently than the wild-type enzyme, while production of 11-hydroxy-ferruginol is abolished. mutant does not oxidize miltiradiene, reaction of EC 1.14.13.190, or manoyl oxide
V296L
-
mutant produces 1.5 times more ferruginol, but 8fold less 11-hydroxy-ferruginol and 20fold less 11beta-hydroxy-manoyl oxide than the wild-type enzyme. mutant does not oxidize miltiradiene, reaction of EC 1.14.13.190