1.14.14.60: ferruginol monooxygenase
This is an abbreviated version!
For detailed information about ferruginol monooxygenase, go to the full flat file.
Reaction
Synonyms
Cyp76AH22, Cyp76AH23, CYP76AH24, CYP76AH3
ECTree
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Engineering
Engineering on EC 1.14.14.60 - ferruginol monooxygenase
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F112L
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mutant catalyzes ferruginol production in yeast 2 times more efficiently than the wild-type enzyme, while production of 11-hydroxy-ferruginol is abolished. mutant does not oxidize miltiradiene, reaction of EC 1.14.13.190, or manoyl oxide
V296L
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mutant produces 1.5 times more ferruginol, but 8fold less 11-hydroxy-ferruginol and 20fold less 11beta-hydroxy-manoyl oxide than the wild-type enzyme. mutant does not oxidize miltiradiene, reaction of EC 1.14.13.190