1.14.14.52: (S)-limonene 7-monooxygenase

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For detailed information about (S)-limonene 7-monooxygenase, go to the full flat file.

Reaction

Synonyms

(-)-limonene 7-monooxygenase, (-)-limonene hydroxylase, (-)-limonene,NADPH:oxygen oxidoreductase (7-hydroxylating, (-)-limonene-7-hydroxylase, EC 1.14.13.49, More, oxygenase, (-)-limonene mono-

ECTree

1 Oxidoreductases

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor

1.14.14.52 (S)-limonene 7-monooxygenase

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Results	in table
2	Activating Compound
87	AA Sequence
1	CAS Registry Number
2	Cloned(Commentary)
4	Cofactor
9	Inhibitors
1	KM Value [mM]
3	Localization
1	Molecular Weight [Da]
4	Natural Substrates/ Products (Substrates)
4	Organism
4	Pathway
9	PDB ID
3	pH Optimum
3	pH Range
1	Protein Variants
1	Purification (Commentary)
2	Reaction
3	Reaction Type
4	Reference
3	Source Tissue
1	Specific Activity [micromol/min/mg]
8	Substrates and Products (Substrate)
2	Subunits
7	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
1	Temperature Stability [°C]

Engineering

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Engineering on EC 1.14.14.52 - (S)-limonene 7-monooxygenase

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additional information

Perilla frutescens

construction of a chimeric mutant enzyme by fusion of the N-terminal membrane insertion domain of the limonene-3-hydroxylase into limonene 7-hydroxylase. The kinetically competent recombinant protein produces a mixture of C3-, C6- and C7-hydroxylated limonene derivatives with a distribution of 33%, 14% and 53%, respectively

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