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1.14.14.3: bacterial luciferase

This is an abbreviated version!
For detailed information about bacterial luciferase, go to the full flat file.

Word Map on EC 1.14.14.3

Reaction

a long-chain aldehyde
+
FMNH2
+
O2
=
a long-chain fatty acid
+
FMN
+
H2O
+
hnu

Synonyms

4a-hydroperoxy-4a,5-dihydroFMN intermediate luciferase, aldehyde monooxygenase, alkanal monooxygenase (FMN), bacterial luciferase, COB, Gluc luciferase, HFOOH, luciferase, Lux, LuxA, LuxAB, LuxB, LuxCDABE, LuxF, Vibrio fischeri luciferase, Vibrio harveyi luciferase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.3 bacterial luciferase

Temperature Stability

Temperature Stability on EC 1.14.14.3 - bacterial luciferase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 37
-
half-life of about 1020 min at 37°C. At 37°C, the native enzyme retains around 60% of the bioluminescence of the enzyme expressed at 30°C
40
-
wild-type enzyme and mutanbt A75G: loss of 60% activity within 50 min, mutants C106V and C106V/A75G show increased thermolability loosing 99% and 90% activity, respectively
47
-
the enzyme is inactivated in 15 min at 47°?
additional information