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1.14.14.3: bacterial luciferase

This is an abbreviated version!
For detailed information about bacterial luciferase, go to the full flat file.

Word Map on EC 1.14.14.3

Reaction

a long-chain aldehyde
+
FMNH2
 +
O2
=
a long-chain fatty acid
 +
FMN
 +
H2O
 +
hnu

Synonyms

4a-hydroperoxy-4a,5-dihydroFMN intermediate luciferase, aldehyde monooxygenase, alkanal monooxygenase (FMN), bacterial luciferase, COB, Gluc luciferase, HFOOH, luciferase, Lux, LuxA, LuxAB, LuxB, LuxCDABE, LuxF, Vibrio fischeri luciferase, Vibrio harveyi luciferase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.3 bacterial luciferase

Purification

Purification on EC 1.14.14.3 - bacterial luciferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
affinity methods
DEAE-Sepharose column chromatography, Superdex 200 gel filtration, and Mono Q column chromatography
-
nickel affinity column
-
on a nickel affinity column, to more than 90% purity
on nickel affinity column, to more than 90% purity
-
preparation of enzyme with modified subunits
-
preparation of subunits
-
recombinant enzyme from Escherichia coli
recombinant enzyme from Escherichia coli strain JM101 to over 95% purity
-
recombinant wild-type and mutant C106A enzymes from Escherichia coli strain JM101 to over 95% purity
-
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21
-
recombinant wild-type and mutant enzymes from Escherichia coli strain JM101 to homogeneity
-
recombinant wild-type and mutant enzymes from Escherichia coli strain JM109 to over 85% homogeneity
-