1.14.14.3: bacterial luciferase
This is an abbreviated version!
For detailed information about bacterial luciferase, go to the full flat file.
Word Map on EC 1.14.14.3
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1.14.14.3
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metastasis
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transwell
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bioluminescence
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chromatin
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lncrnas
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sponge
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tumorigenesis
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endothelial
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3\'utr
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transactivation
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firefly
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3'-untranslated
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necrosis
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agonist
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tnf
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prostate
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estrogen
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sp1
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luminescence
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nude
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nf-kappab
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5'-flanking
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epithelial-mesenchymal
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co-transfection
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cyclin
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glioma
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nsclc
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emsas
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ovarian
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carcinogenesis
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mapks
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pull-down
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hypoxia-inducible
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osteoblast
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erk
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non-small
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bcl-2
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adenovirus
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osteosarcoma
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chemoresistance
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stat3
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pten
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c-jun
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tunel
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e-cadherin
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cardiomyocytes
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transcription-quantitative
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runx2
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osteogenic
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homeobox
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biotechnology
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agriculture
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diagnostics
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medicine
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molecular biology
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analysis
- 1.14.14.3
- metastasis
-
transwell
-
bioluminescence
- chromatin
- lncrnas
- sponge
- tumorigenesis
- endothelial
-
3\'utr
-
transactivation
- firefly
-
3'-untranslated
- necrosis
- agonist
- tnf
- prostate
- estrogen
- sp1
-
luminescence
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nude
- nf-kappab
-
5'-flanking
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epithelial-mesenchymal
-
co-transfection
- cyclin
- glioma
-
nsclc
-
emsas
- ovarian
- carcinogenesis
- mapks
-
pull-down
-
hypoxia-inducible
- osteoblast
- erk
-
non-small
- bcl-2
- adenovirus
- osteosarcoma
-
chemoresistance
- stat3
- pten
- c-jun
-
tunel
- e-cadherin
- cardiomyocytes
-
transcription-quantitative
- runx2
-
osteogenic
-
homeobox
- biotechnology
- agriculture
- diagnostics
- medicine
- molecular biology
- analysis
Reaction
Synonyms
4a-hydroperoxy-4a,5-dihydroFMN intermediate luciferase, aldehyde monooxygenase, alkanal monooxygenase (FMN), bacterial luciferase, COB, Gluc luciferase, HFOOH, luciferase, Lux, LuxA, LuxAB, LuxB, LuxCDABE, LuxF, Vibrio fischeri luciferase, Vibrio harveyi luciferase
ECTree
1.14.14.3 bacterial luciferaseAdvanced search results
results (
results (Natural Substrates Products
Natural Substrates Products on EC 1.14.14.3 - bacterial luciferase
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NATURAL SUBSTRATE 
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
coelenterazine + FMNH2 + O2
CO2 + coelenteramide + FMN + light + H2O
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an imidazolopyrazine derivative
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ir
an aldehyde + FMNH2 + O2
a carboxylate + FMN + H2O + hnu
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-
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?
an aldehyde + FMNH2 + O2
a carboxylate + FMN + H2O + hnu
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-
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?
an aldehyde + FMNH2 + O2
a carboxylate + FMN + H2O + hnu
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-
-
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?
an aldehyde + FMNH2 + O2
a carboxylate + FMN + H2O + hnu
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-
-
-
?
an aldehyde + FMNH2 + O2
a carboxylate + FMN + H2O + hnu
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-
-
-
?
an aldehyde + FMNH2 + O2
a carboxylate + FMN + H2O + hnu
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-
-
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?
an aldehyde + FMNH2 + O2
a carboxylate + FMN + H2O + hnu
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-
-
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?
decanal + FMNH + O2
decanoic acid + FMN + H2O + light
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-
-
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ir
decanal + FMNH + O2
decanoic acid + FMN + H2O + light
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-
-
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ir
decanal + FMNH + O2
decanoic acid + FMN + H2O + light
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-
-
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ir
decanal + FMNH + O2
decanoic acid + FMN + H2O + light
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-
-
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ir
decanal + FMNH + O2
decanoic acid + FMN + H2O + light
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-
-
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ir
decanal + FMNH + O2
decanoic acid + FMN + H2O + light
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348545, 348546, 348547, 348548, 348549, 348550, 348551, 348552, 348553, 348554, 348555, 348557, 348558, 348559, 348560, 348562, 348564, 348565, 348566, 348567, 348568, 348569, 348570, 348571, 348572, 348573, 348574, 348575, 348576, 348577, 348579, 348581, 348582, 348583, 348584, 348585, 348587, 348588, 348589, 348597, 348599, 348600, 348601
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ir
luciferin + O2 + ATP
oxyluciferin + AMP + diphosphate + CO2 + light
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-
-
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ir
luciferin + O2 + ATP
oxyluciferin + AMP + diphosphate + CO2 + light
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-
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ir
RCHO + FMNH2 + O2
RCOOH + FMN + H2O + hn
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3-step process via H2O2 as intermediate
generation of blue-green light of wavelength 490 nm
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ir
RCHO + FMNH2 + O2
RCOOH + FMN + H2O + hn
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3-step process via H2O2 as intermediate
generation of blue-green light of wavelength 490 nm
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ir
RCHO + FMNH2 + O2
RCOOH + FMN + H2O + hn
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long-chain aldehydes
long-chain fatty acids, bioluminescence reaction
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ir
RCHO + FMNH2 + O2
RCOOH + FMN + H2O + hnu
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reduced FMN, i.e. FMNH2, generated by several species of flavin reductases, is utilized along with a long-chain aliphatic aldehyde and molecular oxygen by luciferase as substrates for the bioluminescence reaction, direct transfer of reduced flavin cofactor and reduced flavin product of reductase to luciferase, NADPH-specific FMN reductase and luciferase form a complex in vivo, reduction of reductase-bound FMN cofactor by NADPH is reversible, allowing the cellular contents of NADP+ and NADPH as a factor for the regulation of the production of FMNH2 by FRPVh for luciferase bioluminescence, overview
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?
RCHO + FMNH2 + O2
RCOOH + FMN + H2O + hnu
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reduced FMN, i.e. FMNH2, generated by several species of flavin reductases, is utilized along with a long-chain aliphatic aldehyde and molecular oxygen by luciferase as substrates for the bioluminescence reaction, direct transfer of reduced flavin cofactor and reduced flavin product of reductase to luciferase, NADPH-specific FMN reductase and luciferase form a complex in vivo, reduction of reductase-bound FMN cofactor by NADPH is reversible, allowing the cellular contents of NADP+ and NADPH as a factor for the regulation of the production of FMNH2 by FRPVh for luciferase bioluminescence, overview
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?
additional information
?
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the Gluc luciferase retains its luminescence output in the stationary phase of growth and exhibits enhanced stability during exposure to low pH, hydrogen peroxide, and high temperature
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?
additional information
?
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the decay rate of the enzyme is determined by residue Glu175 of the central region of the LuxA subunit, distinction between slow and fast decay luciferases is primarily due to differences in aldehyde affinity and in the decomposition of the luciferase-flavin-oxygen intermediate
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?
additional information
?
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complex formation in a 1:1 molar ratio between monomeric, but not dimeric, NADPH:FMN oxidoreductase FRP and luciferase for direct transfer of cofactor FMNH2
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?
additional information
?
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the enzyme plays a role in protection of cells against oxidative stress
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-
?
additional information
?
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Vibrio harveyi NADPH-specific flavin reductase FRP transfers reduced riboflavin-5'-phosphate to luciferase by both free diffusion and direct transfer, resulting inbioluminescence production, FRP:luciferase coupled bioluminescence reaction, overview, increases in oxygen concentration lead to gradual decreases of the peak bioluminescence intensity, Km for FMN, and Km for NADPH of NADPH-specific flavin reductase in the coupled reaction with luciferase
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?
