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1.14.14.25: cholesterol 24-hydroxylase

This is an abbreviated version!
For detailed information about cholesterol 24-hydroxylase, go to the full flat file.

Word Map on EC 1.14.14.25

Reaction

cholesterol
+
[reduced NADPH-hemoprotein reductase]
 +
O2
=
(24S)-cholest-5-ene-3beta,24-diol
 +
[oxidized NADPH-hemoprotein reductase]
 +
H2O

Synonyms

24S-hydroxylase, CH24H, cholesterol 24-hydroxylase, cholesterol 24-monooxygenase, cholesterol 24S hydroxylase, cholesterol 24S-hydroxylase, cholesterol hydroxylase, cholesterol-24S-hydroxylase, CYP46, CYP46A, CYP46A1, cytochrome P-450 46A1, cytochrome P450 46A1, cytochrome P450 cholesterol 24-hydroxylase, EC 1.14.13.98

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.25 cholesterol 24-hydroxylase

Cloned

Cloned on EC 1.14.14.25 - cholesterol 24-hydroxylase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
5'-upstream region of human CYP46 gene
-
adeno-associated vectors expressing wild-type (AAV5-wtCYP46A1) or mutated (AAV5-mtCYP46A1) CYP46A1 cDNA tagged with the hemaglutinin epitope injected in hippocampus, frontal, and parietal cortices of both hemispheres of 3-month-old APP23 mice. AAV5-wtCYP46A1 vector injection in the cortex and hippocampus of amyloid precursor protein/presenilin 1 mice. Overexpression in N2a-APP17 cells
-
co-expression of human CYP46 with glial glutamate transporter EAAT2 in primary Rattus norvegicus astrocytes. Quantitative RT-PCR and real-time PCR CYP46 expression analysis
-
DNA and amino acid sequence determnination and analysis, determination of single nucleotide polymorphisms in North American Caucasians and Caribbean Hispanic Alzheimer patients, overview
-
DNA sequence determination and analysis
-
DNA sequence determination and analysis, located on chromosome14q32.1
-
expressed in 293 cells
-
expressed in Escherichia coli
-
expressed in Escherichia coli GC5 cells
expressed in Mus musculus
-
expression Escherichia coli, HEK293 cells transfected with CYP46A1
-
expression in Escherichia coli
-
expression in Escherichia coli, wild-type and trucation mutatants. All four mutants lack the N-terminal transmembrane region (residues 3–27), and, in addition, DELTA46A1 has a 4 His-tag fused to the C-terminus, HDELTA46A1 has the N-terminal 4 His-tag, HDELTA46A1DELTA has a 4 His-tag at the N-terminus and does not contain a proline-rich region at the C-terminus (residues 494–499), and DELTA46A1DELTA lacks the C-terminal proline-rich region
-
full ORF expression clone p46+ including the full cyp46A1 mouse open reading frame. Cyp46A1 overexpressed in 10 days in vitro hippocampal neurons
-
full-length and truncated (DELTA2-50) CYP46A1 expressed in Escherichia coli membranes
gene CYP46A1, CYP46A1 restoration protects from Exp-HTT-induced striatal dysfunctions in vitro. CYP46A1 restoration in the striatum improves the motor phenotype of R6/2 mice
gene CYP46A1, expression analysis
gene CYP46A1, expressionin CHO-K1 cells and HEK-293 cells
-
gene CYP46A1, quantitative expression analysis
gene CYP46A1, recombinant expression of wild-type full-length and mutant truncated forms of enzyme CYP46A1 in Escherichia coli
His-tagged enzyme