Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.14.14.24: vitamin D 25-hydroxylase

This is an abbreviated version!
For detailed information about vitamin D 25-hydroxylase, go to the full flat file.

Word Map on EC 1.14.14.24

Reaction

calciol
+
O2
 +
[reduced NADPH-hemoprotein reductase]
=
calcidiol
 +
[oxidized NADPH-hemoprotein reductase]
 +
H2O

Synonyms

CYP2R1, CYP24A1, CYP2C11, CYP2D25, CYP2J3, CYP2R1, CYPIIJ3, Cytochrome P450 2C11, cytochrome P450 2J2, Cytochrome P450 2J3, cytochrome P450 2R1, EC 1.14.13.159, VDH, vitamin D 25-hydroxylase, vitamin D-25-hydroxylase, vitamin D2 25-hydroxylase, vitamin D3 25-hydroxylase, vitamin D3 hydroxylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.24 vitamin D 25-hydroxylase

Cloned

Cloned on EC 1.14.14.24 - vitamin D 25-hydroxylase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expresion in Escherichia coli
expression in Escherichia coli
expression in HEK 293 cell
expression in insect cells
expression in Saccharomyces cerevisiae
-
functional recombinant expression of human CYP2R1 in Saccharomyces cerevisiae
gene CYP2R1, quantitative real-time PCR enzyme expression analysis
gene CYP2R1, real-time RT-PCR enzyme expression analysis, transient expression of hCYP2R1 promoter (-2 kb)-luciferase reporter plasmid, stimulation with 1,25(OH)2D3 causes a 4.3fold increase in promoter activity. In addition, 1,25-(OH)2D3 significantly increased c-Fos, p-c-Jun expression, and c-Jun N-terminal kinase activity in these cells. AP-1 is a downstream effector of 1,25(OH)2D3 signaling to modulate CYP2R1 gene expression in OSCC tumor cells
transfection into COS-M6 cell
-