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1.14.14.21: dibenzothiophene monooxygenase

This is an abbreviated version!
For detailed information about dibenzothiophene monooxygenase, go to the full flat file.

Word Map on EC 1.14.14.21

Reaction

dibenzothiophene
+ 2 FMNH2 + 2 O2 =
dibenzothiophene-5,5-dioxide
 + 2 FMN + 2 H2O

Synonyms

BdsC, benzothiophene monooxygenase, BT monooxygenase, cofactor-requiring dibenzothiophene monooxygenase, DBT monooxygenase, DBT-MO, DBT-monooxygenase, dibenzothiophene monooxygenase, dszC, TdsC

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.21 dibenzothiophene monooxygenase

Purification

Purification on EC 1.14.14.21 - dibenzothiophene monooxygenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native enzyme 116fold from strain All-2 by two different steps of anion exchange chromatography and dialysis, followed by hydrophobic interaction and hydroxyapatite chromatography
native enzyme 19.2fold from strain WU-S2B by two different steps of anion exchange chromatography and one step of hydrophobic interaction chromatography, recombinant enzyme from Escherichia coli 4.5fold to homogeneity by anion exchange and hydrophobic interaction chromatography
-
native enzyme 24fold to homogeneity from strain D-1 by ammonium sulfate fractionation, anion exchange chromatography, hydrophobic interaction chromatography, and another different step of anion exchange chromatography, followed by gel filtration
native enzyme from strain IGTS8 by dye ligand affinity chromatography, dialysis, and hydrophobic interaction chromatography, followed by gel filtration
native enzyme to homogeneity by hydrophobic interaction and anion exchange chromatography. and gel filtration
recombinant enzyme 39fold from Escherichia coli strain BL21(DE3) by affinity chromatography
recombinant enzyme from Escherichia coli strain BL21 by anion exchange and hydrophobic interaction chromatography, followed by dialysis
recombinant enzyme from Escherichia coli strain BL21(DE3)
recombinant His-tagged enzyme from Escherichia coli strain DH5alpha by nickel affinty chromatography
-
recombinant His-tagged enzyme from Escherichia coli strain Rosetta(DE3) by nickel affinity and anion exchange chromatography, followed by ultrafiltration
recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography, and gel filtration
-
recombinant N-terminally His-tagged enzyms from Escherichia coli strain by nickel affinity and anion exchange chromatography, and gel filtration
recombinant wild-type, selenomethionine-labeled, and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography, and gel filtration