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1.14.14.21: dibenzothiophene monooxygenase

This is an abbreviated version!
For detailed information about dibenzothiophene monooxygenase, go to the full flat file.

Word Map on EC 1.14.14.21

Reaction

dibenzothiophene
+ 2 FMNH2 + 2 O2 =
dibenzothiophene-5,5-dioxide
+ 2 FMN + 2 H2O

Synonyms

BdsC, benzothiophene monooxygenase, BT monooxygenase, cofactor-requiring dibenzothiophene monooxygenase, DBT monooxygenase, DBT-MO, DBT-monooxygenase, dibenzothiophene monooxygenase, dszC, TdsC

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.21 dibenzothiophene monooxygenase

Cloned

Cloned on EC 1.14.14.21 - dibenzothiophene monooxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene bdsC, DNA and amino acid sequence determination and analysis, recombinant functional expression in Escherichia coli strain JM109, coexpression with a flavin reductase Frb, encoded by gene frb, UniProt ID Q75V96. Coexpression of frb with the DBT-desulfurization genes (bdsABC) from Bacillus subtilis strain WU-S2B is critical for high DBT-desulfurizing ability over a wide temperature range of 20-55°C, coexpression screening using indigo production as selective indication
gene bdsC, recombinant expression in Escherichia coli strain BL21(DE3)/pET21-a/bdsC
-
gene bdsC, recombinant overexpression in Escherichia coli
-
gene dszC
gene dszC, cloning from dszABC operon, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis
gene dszC, cloning from strain DS-3, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) and of selenomethionine-labeled enzyme in Escherichia coli strain B834(DE3)
gene dszC, DNA and amino acid sequence determination and analysis, cloning and recombinant expression in Escherichia coli strain JM109
gene dszC, DNA and amino acid sequence determination and analysis, sequence comparison, recombinant expression of His-tagged enzyme in Escherichia coli strain DH5alpha
-
gene dszC, functional recombinant enzyme expression in Thermus thermophilus strain HB27
gene dszC, overexpression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene dszC, phylogenetic analysis, recombinant expression in Escherichia coli strain BL21(DE3)
gene dszC, recombinant expression in Escherichia coli strain BL21(DE3)
gene dszC, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta(DE3)
gene dszC, recombinant expression of the complete dszABC operon, revealing that the ratio of mRNA quantity of dszA, dszB, and dszC is 11:3.3:1, the expression level of dszB is far lower than that of dszC. The termination codon of dszA and the initiation codon of dszB overlap, whereas there is a 13-bp gap between genes dszB and dszC. Quantitative analysis of dsz operon transcription by real-time PCR
gene dszC, sequence comparisons, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
gene sox/dszC, overexpression in Escherichia coli strain BL21
gene tdsC, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, recombinant expression in Escherichia coli, coexpression with genes tdsC and tdsB, leading to established thermophilic desulfurization of dibenzothiophene in Escherichia coli host, an Escherichia coli oxidoreductase can be functionally coupled with the monooxygenases of a gram-positive thermophile