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1.14.14.19: steroid 17alpha-monooxygenase

This is an abbreviated version!
For detailed information about steroid 17alpha-monooxygenase, go to the full flat file.

Word Map on EC 1.14.14.19

Reaction

a C21-steroid
+
[reduced NADPH-hemoprotein reductase]
 +
O2
=
a 17alpha-hydroxy-C21-steroid
 +
[oxidized NADPH-hemoprotein reductase]
 +
H2O

Synonyms

17 alpha-hydroxylase/C17,20-lyase, 17,20-lyase, 17-alpha-hydroxylase/C17-20 lyase, 17-hydroxylase/17,20-lyase, 17-hydroxylase/C17,20-lyase, 17alphahydroxylase, 17alpha-hydroxylase, 17alpha-hydroxylase 17,20 lyase, 17alpha-hydroxylase-17,20-lyase, 17alpha-hydroxylase-C17,20 lyase, 17alpha-hydroxylase-C17,20-lyase, 17alpha-hydroxylase/17,20 lyase, 17alpha-hydroxylase/17,20-lyase, 17alpha-hydroxylase/C(17,20)-lyase, 17alpha-OHase, 17OHD, CYP 17, CYP17, CYP17A1, CYPXVII, cytochrome P-450 (P45017alpha,lyase), cytochrome P-45017alpha, cytochrome P450 17, cytochrome P450 17A1, cytochrome P450 17A2, cytochrome P450 17alpha-hydroxylase, cytochrome P450 17alpha-hydroxylase-17,20-lyase, cytochrome P450 17alpha-hydroxylase/17,20 lyase, cytochrome P450 17alpha-hydroxylase/17,20-lyase, cytochrome p450 17alpha-hydroxylase/C(17,20)-lyase, cytochrome P450 17alpha-hydroxylase/C17,20-lyase, cytochrome P450 17alpha-hydroxylase/c17-20 lyase, cytochrome P45017alpha, cytochrome P450c17, cytochrome P450c17alpha, cytochromeP450 17alpha-hydroxylase/C17-20 lyase, EC 1.14.1.7, EC 1.14.99.9, EC 1.99.1.9, P450 17, P450 17A1, P450(17alpha), P450-17alpha, P450-C17, P45017alpha, P450c17, P450c17-I, Steroid 17-alpha-hydroxylase/17,20 lyase, steroid 17alpha-hydroxylase, steroidogenic cytochrome P450 17A1

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.19 steroid 17alpha-monooxygenase

Purification

Purification on EC 1.14.14.19 - steroid 17alpha-monooxygenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
metal affinity and ion exchange chromatography
native enzyme partially from brain microsomes
-
native enzyme partially from pancreatic cell microsomes
-
Ni-NTA column chromatography, DEAE column chromatography, and SP-Sepharose column chromatography
nickel-nitrilotriacetic acid affinity resin column chromatography, His Select resin column chromatography, and Sartorius Vivapure-S spin column chromatography
nickel-nitrilotriacetic acid-agarose resin column chromatography, carboxymethyl-Sepharose column chromatography, and Superdex 200 gel filtration
purification system especially useful for steroid enzyme assays utilizing radiolabeled substrates
-
recombinant enzyme partially from Pichia pastoris strain GS115 by microsome preparation
-
recombinant fusion enzyme from Escherichia coli by 2',5'-ADP affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain JM109 by nickel affinity chromatography
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain JM109 by anion exchange and nickel affinity chromatography, hydroxyapatite chromatography, and gel filtration
-
recombinant membrane-embedded His-tagged wild-type and mutant enzymes from Escherichia coli strain JM109 by nickel affinity chromatography
recombinant wild-type and mutant enzymes from yeast mcirosomes, recombinant modified CYP17A1 from Escherichia coli strain JM109 to homogeneity