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1.14.14.18: heme oxygenase (biliverdin-producing)

This is an abbreviated version!
For detailed information about heme oxygenase (biliverdin-producing), go to the full flat file.

Word Map on EC 1.14.14.18

Reaction

protoheme
+ 3 [reduced NADPH-hemoprotein reductase] + 3 O2 =
Biliverdin
 +
Fe2+
 +
CO
 + 3 [oxidized NADPH-hemoprotein reductase] + 3 H2O

Synonyms

biliverdin-producing heme oxygenase, ChuS, ChuZ, EC 1.14.99.3, haem oxygenase, heme oxygenase, heme oxygenase 1, heme oxygenase 2, heme oxygenase-1, heme oxygenase-2, HemO, Hmox1, Hmox1a, Hmox1b, Hmox2, Hmox2a, Hmox2b, HmuO, Hmx1, HO, HO-1, HO-2, Ho1, Ho2, Ho3, HO4, HSP32, HugZ, HY1, inducible heme oxygenase-1, More, MsHO1, ORP33 proteins, oxygenase, heme (decyclizing), pbsA1, PigA, proteins, specific or class, ORP33 (oxygen-regulated protein 33,000-mol.-wt.), Syn HO-1, Syn HO-2

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.18 heme oxygenase (biliverdin-producing)

Purification

Purification on EC 1.14.14.18 - heme oxygenase (biliverdin-producing)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 separate fractions after first DEAE-cellulose, hydroxyapatite, Sephadex G-150, DEAE-cellulose
-
ammonium sulfate, DEAE-cellulose, hydroxyapatite, Sephadex G-200
-
ammonium sulfate, Sephadex G-75, DEAE-cellulose, hydroxyapatite, tryptic 28000 Da fragment of recombinant heme oxygenase-2
-
DEAE-cellulose, Sephadex G-200
-
DEAE-ion exchange expanded bed adsorption and Superdex 75 gel filtration, the specific HO-1 activity increases from 0.82 to 24.8 U/mg during purification steps
-
DEAE-Sephacel, carboxymethyl-cellulose, hydroxyapatite, Superose 6/12
-
deletion of 23 C-terminal amino acids, which serve as the membrane-spanning domain, allows for a rapid purification of truncated, soluble HO-1 in large quantities, by standard column chromatography
-
heme oxygenase-1
-
heme oxygenase-2
-
HY1, HO3, and HO4. HO3 and HO4 purified by cobalt affinity chromatography, to over 90% purity
-
hydroxylapatite column chromatography and POROS-HQ column chromatography
Ni-NTA column chromatography and Resource Q column chromatography
-
on anion-exchange column
on Ni-NTA column, wild-type 32fold purified and mutant H25A 31fold purified, more than 95% pure
-
partial
recombinant C-terminal truncated heme oxygenase-1, ammonium sulfate, Mono Q, recombinant heme oxygenase-1/cytochrome P450 reductase fusion protein, ammonium sulfate, 2',5'-ADP-Sepharose
-
recombinant ChuZ from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
-
recombinant GST-tagged full-length HO-1 mutant R254K 54fold and recombinant wild-type enzyme 40fold by thrombin treatment and glutathione affinity chromatography, elution with 1.0% sarkosyl detergent and 2% octyl glucoside, the recombinant wild-type full-length enzyme is not stable during expression and purification due to proteolytic cleavage, overview
recombinant GST-tagged wild-type and mutant HO-2 from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography
recombinant His-tagged HO1 from Escherichia coli by nickel affinity chromatography
recombinant His6-tagged HugZ from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
-
recombinant wild-type and His-tagged enzyme, ion-exchange, gel filtration
-
Sephadex G-75 gel filtration and DE-52 gel filtration
-
Sephadex G25 gel filtration
-
to apparent homogeneity
-
Triton X-100 + cholate, DEAE-cellulose, hydroxyapatite, Sepharose CL-6B, hydroxyapatite
-
truncated HO-1 and HO-2 lacking the 23 C-terminal residues, by gel filtration
-
verdoheme-HmuO complex by anion exchange chromatography and gel filtration