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1.14.14.18: heme oxygenase (biliverdin-producing)

This is an abbreviated version!
For detailed information about heme oxygenase (biliverdin-producing), go to the full flat file.

Word Map on EC 1.14.14.18

Reaction

protoheme
+ 3 [reduced NADPH-hemoprotein reductase] + 3 O2 =
Biliverdin
 +
Fe2+
 +
CO
 + 3 [oxidized NADPH-hemoprotein reductase] + 3 H2O

Synonyms

biliverdin-producing heme oxygenase, ChuS, ChuZ, EC 1.14.99.3, haem oxygenase, heme oxygenase, heme oxygenase 1, heme oxygenase 2, heme oxygenase-1, heme oxygenase-2, HemO, Hmox1, Hmox1a, Hmox1b, Hmox2, Hmox2a, Hmox2b, HmuO, Hmx1, HO, HO-1, HO-2, Ho1, Ho2, Ho3, HO4, HSP32, HugZ, HY1, inducible heme oxygenase-1, More, MsHO1, ORP33 proteins, oxygenase, heme (decyclizing), pbsA1, PigA, proteins, specific or class, ORP33 (oxygen-regulated protein 33,000-mol.-wt.), Syn HO-1, Syn HO-2

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.18 heme oxygenase (biliverdin-producing)

Cloned

Cloned on EC 1.14.14.18 - heme oxygenase (biliverdin-producing)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
265-residue constructs of wild-type and mutant D140A
-
293FT cells transiently cotransfected with a vector containing the viral packaging proteins gag and pol, a vector containing env and pMSCV-scrttaM2puro or pRevTRE-hHO1
-
710-bp fragment encoded amino acids of wild-type and mutant HO-1 constructed in the plasmids pET28b(+)-hHO-1(w) and (m), expressed in Escherichia coli BL21 (DE3)
-
adenovirus-mediated HO-1 transfection of Rattus norvegicus primary cardiomyocytes and H9C2 myocytes
-
DNA and amino acid sequence determination and analysis, genetic structure, phylogenetic analysis, functional expression of His-tagged HO1 in Escherichia coli
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of GFP-tagged HO1 targeted to chloroplasts. Expression of mature MsHO1 in Escherichia coli
DNA and amino acid sequence determination of wild-type and mutant, full-length and truncated enzymes, expression of the N-terminus of the rat enzyme in human HEK-293 cells, and of FLAG-tagged inactive mutant H25A in 3T3 cells, nuclear translocation of enzyme mutants lacking the C-terminus and of mutant H25A, overview
expressed in Escherichia coli
expressed in Escherichia coli BL21 (DE3) cells
-
expressed in Escherichia coli BL21 cells
-
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli DH 5a cells
-
expression analysis
expression in Escherichia coli
expression in Escherichia coli, recombinant protein is inactive
expression in rat basophilic leukemia RBL-2H3 cells
expression in SH-SY5Y cells
expression of C-terminal truncated heme oxygenase-1 and of a heme oxygenase-1 cytochrome P450 reductase fusion protein
-
expression of GST-tagged full-length HO-1 mutant R254K and of GST-tagged wild-type enzyme in Escherichia coli strain DH5alpha
expression of GST-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), transfection of wild-type HO-1 cDNA or intracellular delivery of pure HO-1 protein into cultured NIH 3T3 cells results in activation of a 15-kb HO-1 promoter independently of HO activity, expression of an catalytically inactive HO-1 also results in promoter activation, HO-1-mediated promoter activation is regulated via the distal enhancers E1 and E2
expression of heme oxygenase-2 in Escherichia coli
-
expression of His6-tagged HugZ in Escherichia coli strain BL21(DE3)
-
expression of the kappaB element of the proximal rat HO-1 gene promoter region, -284 to -275, a nuclear target for the NF-kappaB subunit p65/RelA, expression in in RAW264.7 monocytes fused to the luciferase gene. HO-1 promoter activity is induced by p65, an NF-kappaB subunit, which is also termed RelA, and which is a member of the Rel family of proteins and is activated in response to a variety of stimuli
expression of the kappaB element of the proximal rat HO-1 gene promoter region, -284 to -275, a nuclear target for the NF-kappaB subunit p65/RelA, expressionin in RAW264.7 monocytes fused to the luciferase gene
expression of truncated heme oxygenase-1 in Escherichia coli
-
expression of wild-type and His-tagged enzyme in Escherichia coli
-
expression of wild-type heme oxygenase-1 and 2 and heme oxygenase-2 C264A/C281A double mutant in Escherichia coli
-
gene chuZ, ChuZ is highly conserved in 32 clinical isolates. Genotyping, recombinant expression in Escherichia coli strain BL21(DE3)
-
HO-1 expression analysis by real-time PCR
HO-1, DNA and amino acid sequence determination, quantitative expression analysis, overview
HY1, HO3, and HO4 expressed without their predicted chloroplast transit peptides either using a tac promoter-driven N-terminal GST fusion expression system (HY1) or the pET vector system (HO3, HO4) using a T7-promoter and a His-tag fusion. Plasmids pGEX-mHY1, pET24a-mHO3 and pET24a-mHO4 expressed in Escherichia coli BL21 (DE3). Fluorescent protein expression in Nicotiana benthamiana by using Agrobacterium tumefaciens C58C1 pGV2260
-
overexpression of GST-tagged wild-type and mutant HO-2 in Escherichia coli strain BL21(DE3)
overexpression of HO-1 in B16F10 melanoma cells
quantitative enzyme expression analysis
semiquantitative RT-PCR expression analysis of HO-1
sense and antisense constructs, expression analysis in dendritic cells of different maturation levels, cobalt protoporphyrin leads to overexpression of HO-1, molecular mechanism, overview
truncated HO-1 lacking the 23 C-terminal residues, truncated HO-2 lacking the 23 C-terminal residues in the pBAce expression vector construct transformed into Escherichia coli DH5alpha
-
truncated human HO-1 expression vector, expressed in Escherichia coli strain DH5alpha
-
truncated, soluble version of HO-1 that contains 233 amino acids expressed from plasmid HO1-t233/pBace in Escherichia coli DH5alpha