1.14.14.10: nitrilotriacetate monooxygenase

This is an abbreviated version, for detailed information about nitrilotriacetate monooxygenase, go to the full flat file.

Reaction

nitrilotriacetate
+
FMNH2
+
H+
+
O2
=
iminodiacetate
+
glyoxylate
+
FMN
+
H2O

Synonyms

component B of NTA monooxygenase, EDTA monooxygenase, nitrilotriacetate monooxygenase component A, nitrilotriacetate monooxygenase component B, NTA MO A, NTA monooxygenase, NTA-MO, NTA-MoB, NTA-monooxygenase, NtaA

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.10 nitrilotriacetate monooxygenase

Storage Stability

Storage Stability on EC 1.14.14.10 - nitrilotriacetate monooxygenase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-70°C, crude cell extract, up to 1 month, no loss of activity
-
-70°C, purified component B of NTA monooxygenase in 20 mM HEPES buffer with 2 mM dithiothreitol, at pH 7.8, several months, without any significant loss of activity
-
-80°C, partially purified, dialyzed enzyme, 3 months, no loss of activity
-
4°C, crude cell extract, 48 hours, 50% loss of activity
-
4°C, crude extract, 50 mM Tris-HCl, 200 h, 50% loss of activity
-
4°C, purified component B of NTA monooxygenase in 20 mM HEPES buffer at pH 7.8, 7 days, 70% loss of activity
-
4°C, purified component B of NTA monooxygenase in 20 mM HEPES buffer with 2 mM dithiothreitol and ammonium sulfate to 5% saturation, at pH 7.8, 7 days, 5% loss of activity
-
4°C, purified component B of NTA monooxygenase in 20 mM HEPES buffer with 2 mM dithiothreitol, at pH 7.8, 7 days, 20% loss of activity
-