1.14.13.82: vanillate monooxygenase

This is an abbreviated version!
For detailed information about vanillate monooxygenase, go to the full flat file.

Reaction

Synonyms

4-hydroxy-3-methoxybenzoate demethylase, EC 1.2.3.12, IvaAB, More, VAN, VanA, vanAB, VanB, vanillate demethylase, vanillate O-demethylase, vanillate O-demethylase oxygenase, vanillate-O-demethylase, vanillic acid O-demethylase

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

                1.14.13.82 vanillate monooxygenase

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Results	in table
1	Activating Compound
859	AA Sequence
1	CAS Registry Number
10	Cloned(Commentary)
13	Cofactor
1	Expression
4	General Information
3	Inhibitors
1	Metals/Ions
1	Molecular Weight [Da]
29	Natural Substrates/ Products (Substrates)
30	Organism
3	Pathway
9	Protein Variants
1	Reaction
3	Reaction Type
23	Reference
9	Source Tissue
1	Specific Activity [micromol/min/mg]
54	Substrates and Products (Substrate)
4	Subunits
39	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]

Engineering

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Engineering on EC 1.14.13.82 - vanillate monooxygenase

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

H156R

Acinetobacter sp.

-

substituting an iron-binding ligand

639289

W217R

Acinetobacter sp.

-

functional at 22øC but not at 37øC

639289

H156R

Acinetobacter sp. ADP1

-

substituting an iron-binding ligand

-

W217R

Acinetobacter sp. ADP1

-

functional at 22øC but not at 37øC

-

additional information

5 entries

additional information

Acinetobacter sp.

-

several amino acid substitutions give indications of the extent to which amino acid substitutions can be tolerated at specified positions

639289

additional information

Acinetobacter sp. ADP1

-

several amino acid substitutions give indications of the extent to which amino acid substitutions can be tolerated at specified positions

-

additional information

Corynebacterium efficiens

Q8FRX4; Q8FRX3

generation of recombinant Corynebacterium glutamicum strain F, overexpression of vanillate O-demethylase (VanAB) to increase the production of protocatechiuc acid from ferulic acid. The strain FVan produces vanillate from ferulic acid and the enzyme converts it to protocatechiuc acid, a precursor of polymers and plastics and a constituent of food, fed-batch biotransformation, protocatechiuc acid is secreted to the medium, overview

743997

additional information

Corynebacterium efficiens NBRC 100395

-

generation of recombinant Corynebacterium glutamicum strain F, overexpression of vanillate O-demethylase (VanAB) to increase the production of protocatechiuc acid from ferulic acid. The strain FVan produces vanillate from ferulic acid and the enzyme converts it to protocatechiuc acid, a precursor of polymers and plastics and a constituent of food, fed-batch biotransformation, protocatechiuc acid is secreted to the medium, overview

-

additional information

Pseudomonas putida

-

the reaction of the recombinant enzyme is one of the limiting steps of the prospective bioconversion from lignin-derived compounds to valuable products in recombinant Escherichia coli

673722