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1.14.13.7: phenol 2-monooxygenase (NADPH)

This is an abbreviated version!
For detailed information about phenol 2-monooxygenase (NADPH), go to the full flat file.

Word Map on EC 1.14.13.7

Reaction

phenol
+
NADPH
 +
H+
 +
O2
=
catechol
 +
NADP+
 +
H2O

Synonyms

DmpLNO, flavin containing monooxygenase, LmPH, Mph, MphN, multi-component phenol hydroxylase, multicomponent PH, multicomponent phenol hydroxylase, multicomponent phenol hydroxylase alpha subunit, NCgl2588, oxygenase, phenol 2-mono-, PHE, phenol hydroxylase, phenol o-hydroxylase, PHH, phhY, PHIND, PHO, PHR, single-component PH, SPH

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.7 phenol 2-monooxygenase (NADPH)

Cloned

Cloned on EC 1.14.13.7 - phenol 2-monooxygenase (NADPH)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a 7.6 kb SalI fragment of pZC1115 containing LmPH gene subcloned to pUC19 vector, resulting in recombinant plasmid pME08. Expressed in Escherichia coli DH5alpha
co-expression of phenol hydroxylase with the regulatory protein in Escherichia coli JM109, expression of selenomethionine-enzyme in Escherichia coli strain BL21(DE3)
-
co-expression with wild-type or E103 mutant toluene o-xylene monooxygenases in Escherichia coli strain JM109
-
DNA and amino acid sequence determination and analysis of diverse strains and their genes encoding the enzyme, phylogenetic analysis, sequence comparisons, overview
-
DNA and amino acid sequence determination and analysis, expression analysis
-
expressed in Escherichia coli
-
expressed in Escherichia coli BL21(DE3) cells
expression in Escherichia coli LE392
expression in Pseudomonas aeruginosa PAO1c
-
expression of enzyme from strain KL33 and KL28 in Escherichia coli
-
expression of the phenol hydroxylase gene cluster in Escherichia coli strain JM109 in the absence or presence of the phk gene
-
gene pheBA, DNA and amino acid sequence determination and analysis, expression analysis
gene phyA, DNA and amino acid sequence determinations from different strains and analysis, expression analysis, expression of the enzymes from strains R57 and ATCC 46490 in Saccharomyces cerevisiae
-
oxygenase component PHO
-
the enzyme is encoded in the ph operon, expression in Escherichia coli strain JM109
-