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1.14.13.6: orcinol 2-monooxygenase

This is an abbreviated version!
For detailed information about orcinol 2-monooxygenase, go to the full flat file.

Word Map on EC 1.14.13.6

Reaction

orcinol
+
NADH
+
H+
+
O2
=
2,3,5-Trihydroxytoluene
+
NAD+
+
H2O

Synonyms

orcinol hydroxylase, oxygenase, orcinol 2-mono

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.6 orcinol 2-monooxygenase

Purification

Purification on EC 1.14.13.6 - orcinol 2-monooxygenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ORC, using protamine sulfate treatment, column chromatography on DEAE-cellulose, Sephadex G-100, Sephadex G-75 and a second DEAE-cellulose column chromatography
-
using protamine sulfate treatment, column chromatography on DEAE-cellulose, Sephadex G-100, Sephadex G-75, a second DEAE-cellulose column chromatography and crystallization
-
using protamine sulfate treatment, DEAE-cellulose column chromatography, ammonium sulfate treatment and column chromatography on Sephadex G-100 followed by ammonium sulfate treatment and column chromatography on hydroxylapatite
-