1.14.13.59: L-lysine N6-monooxygenase (NADPH)

This is an abbreviated version!
For detailed information about L-lysine N6-monooxygenase (NADPH), go to the full flat file.

Word Map on EC 1.14.13.59

1.14.13.59

fad

siderophore

flavoproteins

ornithine

nicotinamide

c4a-hydroperoxyflavin

hydroxamate

nadp+

hydroxamate-containing

oxygen-dependent

5-aminovalerate

aerobactin

glutarate

semialdehyde

flavin-containing

iodoacetate

fumigatus

Reaction

Synonyms

EC 1.13.12.10, EC 13.12.10, flavin-dependent lysine monooxygenase, flavin-dependent N6-lysine monooxygenase, IucD, LH, lysine monooxygenase, Lysine N(6)-hydroxylase, Lysine N6-hydroxylase, lysine N6-monooxygenase, lysine: N6-hydroxylase, lysine:N(6)-hydroxylase, Lysine:N6-hydroxylase, MbsG, N-hydroxylating monooxygenase, NbtG, Oxygenase, lysine N6-mono-

ECTree

1 Oxidoreductases

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

1.14.13.59 L-lysine N6-monooxygenase (NADPH)

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Results	in table
4	Activating Compound
6102	AA Sequence
1	CAS Registry Number
5	Cloned(Commentary)
36	Cofactor
1	Crystallization (Commentary)
4	General Information
1	General Stability
10	Inhibitors
20	kcat/KM [mM/s]
1	Ki Value [mM]
38	KM Value [mM]
5	Localization
2	Molecular Weight [Da]
8	Natural Substrates/ Products (Substrates)
19	Organism
8	Pathway
4	PDB ID
7	pH Optimum
2	pH Range
25	Protein Variants
4	Purification (Commentary)
5	Reaction
2	Reaction Type
17	Reference
9	Specific Activity [micromol/min/mg]
1	Storage Stability
47	Substrates and Products (Substrate)
5	Subunits
29	Synonyms
1	Systematic Name
7	Temperature Optimum [°C]
28	Turnover Number [1/s]

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Reference on EC 1.14.13.59 - L-lysine N6-monooxygenase (NADPH)

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207904

Plattner, H.J.; Pfefferle, P.; Romaguera, A.; Waschutza, S.; Dieckman, H.

Isolation and some properties of lysine N6-hydroxylase from Escherichia coli strain EN222

Biol. Met.

1-5

1989

Escherichia coli, Escherichia coli EN222

2518519

207905

Macheroux, P.; Plattner, H.J.; Romaguera, A.; Diekmann, H.

FAD and substrate analogs as probes for lysine N6-hydroxylase from Escherichia coli EC 222

Eur. J. Biochem.

213

995-1002

1993

Escherichia coli

8504838

207906

Thariath, A.; Socha, D.; Valvano, M.A.; Viswanatha, T.

Construction and biochemical characterization of recombinant cytoplasmic forms of IucD protein (lysine:N6-hydroxylase) encoded by the pColV-K30 aerobactin gene cluster

J. Bacteriol.

175

589-596

1993

Escherichia coli

8423134

207907

Thariath, A.M.; Fatum, K.L.; Valvano, M.A.; Viswanatha, T.

Physico-chemical characterization of a recombinant cytoplasmic form of lysine:N6-hydroxylase

Biochim. Biophys. Acta

1203

27-35

1993

Escherichia coli

8218389

207908

Marrone, L.; Beecroft, M.; Viswanatha, T.

Lysine:N6-hydroxylase: cofactor interactions

Bioorg. Chem.

304-317

1996

Escherichia coli

207909

Marrone, L.; Viswanatha, T.

Effect of selective cysteine --> alanine replacements on the catalytic functions of lysine:N6-hydroxylase

Biochim. Biophys. Acta

1343

263-277

1997

Escherichia coli

9434117

658171

Dick, S.; Siemann, S.; Frey, H.E.; Lepock, J.R.; Viswanatha, T.

Recombinant lysine:N(6)-hydroxylase: effect of cysteine-->alanine replacements on structural integrity and catalytic competence

Biochim. Biophys. Acta

1594

219-233

2002

Escherichia coli

11904218

658232

Stehr, M.; Smau, L.; Singh, M.; Seth, O.; Macheroux, P.; Ghisla, S.; Diekmann, H.

Studies with lysine N6-hydroxylase. Effect of a mutation in the assumed FAD binding site on coenzyme affinities and on lysine hydroxylating activity

Biol. Chem.

380

47-54

1999

Escherichia coli, Escherichia coli EN222

10064136

659801

Dick, S.; Marrone, L.; Duewel, H.; Beecroft, M.; McCourt, J.; Viswanatha, T.

Lysine: N6-hydroxylase: stability and interaction with ligands

J. Protein Chem.

893-903

1999

Escherichia coli

10839627

726965

Robinson, R.; Sobrado, P.

Substrate binding modulates the activity of Mycobacterium smegmatis G, a flavin-dependent monooxygenase involved in the biosynthesis of hydroxamate-containing siderophores

Biochemistry

8489-8496

2011

Mycolicibacterium smegmatis

21870809

744158

Robinson, R.M.; Rodriguez, P.J.; Sobrado, P.

Mechanistic studies on the flavin-dependent N6-lysine monooxygenase MbsG reveal an unusual control for catalysis

Arch. Biochem. Biophys.

550-551

58-66

2014

Mycolicibacterium smegmatis, Mycolicibacterium smegmatis G

24769337

744167

Abdelwahab, H.; Robinson, R.; Rodriguez, P.; Adly, C.; El-Sohaimy, S.; Sobrado, P.

Identification of structural determinants of NAD(P)H selectivity and lysine binding in lysine N(6)-monooxygenase

Arch. Biochem. Biophys.

606

180-188

2016

Nocardia farcinica (Q5Z1T5), Nocardia farcinica IFM 10152 (Q5Z1T5)

27503802

745322

Binda, C.; Robinson, R.M.; Martin Del Campo, J.S.; Keul, N.D.; Rodriguez, P.J.; Robinson, H.H.; Mattevi, A.; Sobrado, P.

An unprecedented NADPH domain conformation in lysine monooxygenase NbtG provides insights into uncoupling of oxygen consumption from substrate hydroxylation

J. Biol. Chem.

290

12676-12688

2015

Nocardia farcinica

25802330