1.14.13.59: L-lysine N6-monooxygenase (NADPH)

This is an abbreviated version!
For detailed information about L-lysine N6-monooxygenase (NADPH), go to the full flat file.

Reaction

Synonyms

EC 1.13.12.10, EC 13.12.10, flavin-dependent lysine monooxygenase, flavin-dependent N6-lysine monooxygenase, IucD, LH, lysine monooxygenase, Lysine N(6)-hydroxylase, Lysine N6-hydroxylase, lysine N6-monooxygenase, lysine: N6-hydroxylase, lysine:N(6)-hydroxylase, Lysine:N6-hydroxylase, MbsG, N-hydroxylating monooxygenase, NbtG, Oxygenase, lysine N6-mono-

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

                1.14.13.59 L-lysine N6-monooxygenase (NADPH)

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

Results	in table
4	Activating Compound
6102	AA Sequence
1	CAS Registry Number
5	Cloned(Commentary)
36	Cofactor
1	Crystallization (Commentary)
4	General Information
1	General Stability
10	Inhibitors
20	kcat/KM [mM/s]
1	Ki Value [mM]
38	KM Value [mM]
5	Localization
2	Molecular Weight [Da]
8	Natural Substrates/ Products (Substrates)
19	Organism
8	Pathway
4	PDB ID
7	pH Optimum
2	pH Range
25	Protein Variants
4	Purification (Commentary)
5	Reaction
2	Reaction Type
17	Reference
9	Specific Activity [micromol/min/mg]
1	Storage Stability
47	Substrates and Products (Substrate)
5	Subunits
29	Synonyms
1	Systematic Name
7	Temperature Optimum [°C]
28	Turnover Number [1/s]

Crystallization

print visible entries

print all entries

Crystallization on EC 1.14.13.59 - L-lysine N6-monooxygenase (NADPH)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide Go to Crystallization (commentary) Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

purified recombinant detagged wild-type and seleno-L-methionine-labeled enzyme mutant K184A, method screening, drop vapor diffusion method, mixing of 0.001 ml of 6 mg/ml protein in 25 mM HEPES/NaOH, pH 7.5, 500 mM NaCl, and 5 mM NADP+ in the presence of either L-Lys or D-Lys, with 0.001 ml of reservoir solution containing 10% w/v PEG 6000, 5% v/v 2,4-methylpentanediol, and 0.1 M HEPES/NaOH, pH 7.5, 3-4 days at 20°C, micro-seeding technique, X-ray diffraction structure determination and analysis at 2.4-2.9 A resolution

Nocardia farcinica

-

745322