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1.14.13.50: pentachlorophenol monooxygenase

This is an abbreviated version!
For detailed information about pentachlorophenol monooxygenase, go to the full flat file.

Word Map on EC 1.14.13.50

Reaction

2,3,5,6-Tetrachlorophenol
+
NADPH
+
H+
+
O2
=
2,3,5,6-tetrachlorohydroquinone
+
NADP+
+
H2O

Synonyms

CpsB, dechlorinase, pentachlorophenol, GM610_18120, oxygenase, pentachlorophenol 4-mono-, PCB hydroxylase, PCP 4-monooxygenase, PCP hydroxylase, PCP-4-monooxygenase, PCP-monooxygenase, PCP4MO, PcpB, PcpD, pentachlorophenol 4-mono-oxygenase, pentachlorophenol 4-monooxygenase, pentachlorophenol dechlorinase, pentachlorophenol dehalogenase, pentachlorophenol hydroxylase, pentachlorophenol monooxygenase, pentachlorophenol-4-monooxygenase, Phe4MO, tetrachlorobenzoquinone reductase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.50 pentachlorophenol monooxygenase

Engineering

Engineering on EC 1.14.13.50 - pentachlorophenol monooxygenase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
F223A
-
site-directed mutagenesis, increased activity compared to the wild-type enzyme
F225A
-
site-directed mutagenesis, increased activity compared to the wild-type enzyme
F87A
-
site-directed mutagenesis, mutant enzyme is nearly not soluble in contrast to the wild-type, highly reduced activity compared to the wild-type enzyme
moe
-
construction of an allelic exchange mutant and a pcpD knockout mutant strains, the latter show reduced activity at high pentachlorophenol concentrations, not at low concentrations
R235A
-
site-directed mutagenesis, determination of molecular weight and comparison to wild-type enzyme
R235E
R235K
Y216A
Y216F
-
site-directed mutagenesis, 60% decreased activity compared to the wild-type enzyme
Y397A
Y397F
additional information