1.14.13.50: pentachlorophenol monooxygenase
This is an abbreviated version!
For detailed information about pentachlorophenol monooxygenase, go to the full flat file.
Word Map on EC 1.14.13.50
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1.14.13.50
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pcp-degrading
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flavobacterium
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sphingobium
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chlorophenolicum
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tetrachlorohydroquinone
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bioremediation
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tetrachlorobenzoquinone
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dehalogenation
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environmental protection
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arthrobacter
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degradation
- 1.14.13.50
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pcp-degrading
- flavobacterium
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sphingobium
- chlorophenolicum
- tetrachlorohydroquinone
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bioremediation
- tetrachlorobenzoquinone
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dehalogenation
- environmental protection
- arthrobacter
- degradation
Reaction
Synonyms
CpsB, dechlorinase, pentachlorophenol, GM610_18120, oxygenase, pentachlorophenol 4-mono-, PCB hydroxylase, PCP 4-monooxygenase, PCP hydroxylase, PCP-4-monooxygenase, PCP-monooxygenase, PCP4MO, PcpB, PcpD, pentachlorophenol 4-mono-oxygenase, pentachlorophenol 4-monooxygenase, pentachlorophenol dechlorinase, pentachlorophenol dehalogenase, pentachlorophenol hydroxylase, pentachlorophenol monooxygenase, pentachlorophenol-4-monooxygenase, Phe4MO, tetrachlorobenzoquinone reductase
ECTree
Advanced search results
Engineering
Engineering on EC 1.14.13.50 - pentachlorophenol monooxygenase
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F223A
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site-directed mutagenesis, increased activity compared to the wild-type enzyme
F225A
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site-directed mutagenesis, increased activity compared to the wild-type enzyme
F85A
F87A
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site-directed mutagenesis, mutant enzyme is nearly not soluble in contrast to the wild-type, highly reduced activity compared to the wild-type enzyme
moe
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construction of an allelic exchange mutant and a pcpD knockout mutant strains, the latter show reduced activity at high pentachlorophenol concentrations, not at low concentrations
R235A
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site-directed mutagenesis, determination of molecular weight and comparison to wild-type enzyme
R235E
R235K
Y216A
Y216F
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site-directed mutagenesis, 60% decreased activity compared to the wild-type enzyme
Y397A
Y397F
additional information
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site-directed mutagenesis, 60% decreased activity compared to the wild-type enzyme
F85A
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site-directed mutagenesis, determination of molecular weight and comparison to wild-type enzyme
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site-directed mutagenesis, reduced activity compared to the wild-type enzyme
R235E
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site-directed mutagenesis, determination of molecular weight and comparison to wild-type enzyme
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site-directed mutagenesis, increased activity compared to the wild-type enzyme
R235K
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site-directed mutagenesis, determination of molecular weight and comparison to wild-type enzyme
R235K
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site-directed mutagenesis, mutant enzyme is nearly not soluble in contrast to the wild-type, reduced activity compared to the wild-type enzyme
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site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme
Y216A
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site-directed mutagenesis, determination of molecular weight and comparison to wild-type enzyme
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site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme
Y397A
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site-directed mutagenesis, determination of molecular weight and comparison to wild-type enzyme
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site-directed mutagenesis, 60% increased activity compared to the wild-type enzyme
Y397F
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site-directed mutagenesis, determination of molecular weight and comparison to wild-type enzyme
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introduction of tobacco etch virus protease cleavage site into the enzyme as a glutathione-S-transferase fusion protein
additional information
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targeted mutagenesis via allelic exchange, construction of isogenic mutant without PCP 4-monooxygenase activity and 2 cointegration mutants with slow activity