1.14.13.4: melilotate 3-monooxygenase

This is an abbreviated version!
For detailed information about melilotate 3-monooxygenase, go to the full flat file.

Reaction

Synonyms

2-hydroxyphenylpropionate hydroxylase, 2-hydroxyphenylpropionic hydroxylase, melilotate hydroxylase, melilotic hydroxylase, oxygenase, melilotate 3-mono-

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

                1.14.13.4 melilotate 3-monooxygenase

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1	CAS Registry Number
21	Cofactor
7	Inhibitors
10	KM Value [mM]
5	Molecular Weight [Da]
1	Natural Substrates/ Products (Substrates)
10	Organism
1	Oxidation Stability
3	Pathway
1	pH Optimum
1	pH Range
3	Purification (Commentary)
6	Reaction
3	Reaction Type
10	Reference
1	Renatured (Commentary)
2	Specific Activity [micromol/min/mg]
3	Storage Stability
12	Substrates and Products (Substrate)
2	Subunits
5	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
1	Turnover Number [1/s]

Substrates Products

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Substrates Products on EC 1.14.13.4 - melilotate 3-monooxygenase

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

3-(2-hydroxyphenyl)propanoate + NADH + O2

3-(2,3-dihydroxyphenyl)propanoate + NAD+ + H2O

11 entries

additional information

?

-

Arthrobacter sp.

-

high specificity, low activity with 3-hydroxyphenylpropanoate with 20% of melilotate reduction, phenylpropanoate with 1-2% of meliotate reduction

396422

-

-

?

3-(2-hydroxyphenyl)propanoate + NADH + O2

3-(2,3-dihydroxyphenyl)propanoate + NAD+ + H2O

Arthrobacter sp.

-

-

390102

-

390102

?

3-(2-hydroxyphenyl)propanoate + NADH + O2

3-(2,3-dihydroxyphenyl)propanoate + NAD+ + H2O

Arthrobacter sp.

-

-

396420

-

396420

?

3-(2-hydroxyphenyl)propanoate + NADH + O2

3-(2,3-dihydroxyphenyl)propanoate + NAD+ + H2O

Arthrobacter sp.

-

i.e. melilotate, reversibility could not be demonstrated

396422

reversibility could not be demonstrated

396422

ir

3-(2-hydroxyphenyl)propanoate + NADH + O2

3-(2,3-dihydroxyphenyl)propanoate + NAD+ + H2O

Arthrobacter sp.

-

i.e. melilotate, role in metabolism of coumarin

396422

-

396422

ir

3-(2-hydroxyphenyl)propanoate + NADH + O2

3-(2,3-dihydroxyphenyl)propanoate + NAD+ + H2O

Pseudomonas sp.

-

-

390102

-

390102

?

3-(2-hydroxyphenyl)propanoate + NADH + O2

3-(2,3-dihydroxyphenyl)propanoate + NAD+ + H2O

Pseudomonas sp.

-

-

396421

-

396421

?

3-(2-hydroxyphenyl)propanoate + NADH + O2

3-(2,3-dihydroxyphenyl)propanoate + NAD+ + H2O

Pseudomonas sp.

-

-

396423

-

396423

?

3-(2-hydroxyphenyl)propanoate + NADH + O2

3-(2,3-dihydroxyphenyl)propanoate + NAD+ + H2O

Pseudomonas sp.

-

-

396424

-

396424

?

3-(2-hydroxyphenyl)propanoate + NADH + O2

3-(2,3-dihydroxyphenyl)propanoate + NAD+ + H2O

Pseudomonas sp.

-

-

396425

-

396425

?

3-(2-hydroxyphenyl)propanoate + NADH + O2

3-(2,3-dihydroxyphenyl)propanoate + NAD+ + H2O

Pseudomonas sp.

-

-

396426

-

396426

?

3-(2-hydroxyphenyl)propanoate + NADH + O2

3-(2,3-dihydroxyphenyl)propanoate + NAD+ + H2O

Pseudomonas sp.

-

-

396427

-

396427

?