1.14.13.29: 4-nitrophenol 2-monooxygenase

This is an abbreviated version!
For detailed information about 4-nitrophenol 2-monooxygenase, go to the full flat file.

Reaction

Synonyms

4-nitrophenol 4-monooxygenase/4-nitrocatechol 2-monooxygenase, 4-nitrophenol hydroxylase, 4-nitrophenol-2-hydroxylase, 4-NPH, CYP 2E1, CYP2A/2E1, CYP2E1, cytochrome P-450 2E1, cytochrome P-450 isozyme 3a, NphA1, oxygenase, 4-nitrophenol 2-mono-, p-nitrophenol 2-hydroxylase, p-nitrophenol hydroxylase, p-nitrophenol hydroxylase component A, p-nitrophenol-hydroxylase, PNP 2-monooxygenase, PNP monooxygenase, PnpA1, PNPH, PPH, two-component 4-nitrophenol hydroxylase, two-component PNP monooxygenase

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

                1.14.13.29 4-nitrophenol 2-monooxygenase

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Results	in table
4	Activating Compound
57	AA Sequence
1	Application
1	CAS Registry Number
4	Cloned(Commentary)
17	Cofactor
2	Crystallization (Commentary)
131	Disease/ Diagnostics
28	General Information
1	General Stability
4	IC50 Value
27	Inhibitors
2	Ki Value [mM]
4	KM Value [mM]
11	Localization
4	Molecular Weight [Da]
13	Natural Substrates/ Products (Substrates)
1	Organic Solvent Stability
26	Organism
3	Pathway
7	pH Optimum
21	Protein Variants
5	Purification (Commentary)
4	Reaction
3	Reaction Type
23	Reference
16	Source Tissue
15	Specific Activity [micromol/min/mg]
2	Storage Stability
45	Substrates and Products (Substrate)
10	Subunits
40	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
1	Turnover Number [1/s]

Crystallization

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Crystallization on EC 1.14.13.29 - 4-nitrophenol 2-monooxygenase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

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Rhodococcus opacus

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763974

purified recombinant His-tagged wild-type enzyme, sitting drop vapor diffusion method, mixing equal volumes of protein solution and reservoir solution. The reservoir solution contains 0.1 M Tris, pH 7.25, 1.5 M (NH4)2SO4, and 4% v/v glycerol, diffraction-quality crystals of PnpA1 appear within 5 days at 20°C, X-ray diffraction structure determination and analysis at 2.5 A resolution

Rhodococcus opacus

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763974