1.14.13.29: 4-nitrophenol 2-monooxygenase
This is an abbreviated version!
For detailed information about 4-nitrophenol 2-monooxygenase, go to the full flat file.
Word Map on EC 1.14.13.29
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1.14.13.29
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cyp2e1
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n-demethylase
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ethoxyresorufin
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cyp1a2
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aniline
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pentoxyresorufin
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prod
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o-dealkylase
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diallyl
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n-nitrosodimethylamine
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chlorzoxazone
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aminopyrine
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o-depentylase
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4-methylpyrazole
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cyp2e1-dependent
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benzphetamine
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benzyloxyresorufin
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cyp2e1-mediated
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acetone-induced
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cyp-dependent
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o-deethylation
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2e1-dependent
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7-ethoxyresorufin-o-deethylase
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ethoxycoumarin
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medicine
- 1.14.13.29
- cyp2e1
- n-demethylase
-
ethoxyresorufin
- cyp1a2
- aniline
-
pentoxyresorufin
-
prod
-
o-dealkylase
-
diallyl
- n-nitrosodimethylamine
- chlorzoxazone
- aminopyrine
-
o-depentylase
- 4-methylpyrazole
-
cyp2e1-dependent
- benzphetamine
-
benzyloxyresorufin
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cyp2e1-mediated
-
acetone-induced
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cyp-dependent
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o-deethylation
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2e1-dependent
- 7-ethoxyresorufin-o-deethylase
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ethoxycoumarin
- medicine
Reaction
Synonyms
4-nitrophenol 4-monooxygenase/4-nitrocatechol 2-monooxygenase, 4-nitrophenol hydroxylase, 4-nitrophenol-2-hydroxylase, 4-NPH, CYP 2E1, CYP2A/2E1, CYP2E1, cytochrome P-450 2E1, cytochrome P-450 isozyme 3a, NphA1, oxygenase, 4-nitrophenol 2-mono-, p-nitrophenol 2-hydroxylase, p-nitrophenol hydroxylase, p-nitrophenol hydroxylase component A, p-nitrophenol-hydroxylase, PNP 2-monooxygenase, PNP monooxygenase, PnpA1, PNPH, PPH, two-component 4-nitrophenol hydroxylase, two-component PNP monooxygenase
ECTree
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Cloned
Cloned on EC 1.14.13.29 - 4-nitrophenol 2-monooxygenase
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DNA and amino acid sequence determination and analysis, phylogenetic analysis
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PCR-amplification, shuttle vector pK4 is used to construct pKPN plasmids containing enzyme gene and reporter gene, hosts are Rhodococcus sp. PN1 and Rhodococcus rhodochrous ATCC 12674 (electroporation), Escherichia coli JM109 is host for propagation and purification of recombinant plasmids
recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain Rosetta(DE3)/pLysS
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