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1.14.13.25: methane monooxygenase (soluble)

This is an abbreviated version!
For detailed information about methane monooxygenase (soluble), go to the full flat file.

Word Map on EC 1.14.13.25

Reaction

methane
+
NAD(P)H
 +
H+
 +
O2
=
methanol
 +
NAD(P)+
 +
H2O

Synonyms

chcA, cytoplasmic methane monooxygenase, methane hydroxylase, methane mono-oxygenase, methane monooxygenase, methane monooxygenase hydroxylase, MmMmoC, MMO, MMO Bath, MMOB, MmoC, MMOH, MMOR, oxygenase, methane mono-, particulate methane monooxygenase, pMMO, sMMO, soluble methane monooxygenase, soluble methane monooxygenase hydroxylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.25 methane monooxygenase (soluble)

General Stability

General Stability on EC 1.14.13.25 - methane monooxygenase (soluble)

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
5 mM thioglycollate, 5 mM dithiothreitol, 5 mM NADH stabilize component C at 0°C
-
Fe2+ stabilizes component C of sMMO
-
instability of enzyme in crude extract
instability of the enzyme during purification is probably due to loss of iron
-
no component of the enzyme is stable to freezing
-
protein A unstable to successive freezing and thawing
-
protein B stable to successive freezing and thawing
-
protein B, in crude form requires addition of protease inhibitor phenylmethylsulfonyl fluoride
-
protein C requires presence of thiol protective agent, e.g. sodium thioglycolate throughout purification
-
sMMO is more stable and easier to purify than pMMO
-