1.14.13.242: 3-hydroxy-2-methylpyridine-5-carboxylate monooxygenase

This is an abbreviated version!
For detailed information about 3-hydroxy-2-methylpyridine-5-carboxylate monooxygenase, go to the full flat file.

Reaction

Synonyms

2-methyl-3-hydroxypyridine 5-carboxylic acid dioxygenase, 2-methyl-3-hydroxypyridine 5-carboxylic acid oxygenase, 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase, 3-hydroxy-2-methylpyridine carboxylate dioxygenase, 3-hydroxy-2-methylpyridinecarboxylate dioxygenase, EC 1.14.12.4, methylhydroxypyridine carboxylate dioxygenase, methylhydroxypyridinecarboxylate oxidase, MHPCO, mlr6788

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

                1.14.13.242 3-hydroxy-2-methylpyridine-5-carboxylate monooxygenase

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Results	in table
7	AA Sequence
1	CAS Registry Number
6	Cloned(Commentary)
22	Cofactor
8	Crystallization (Commentary)
10	General Information
1	IC50 Value
8	Inhibitors
3	kcat/KM [mM/s]
2	Ki Value [mM]
21	KM Value [mM]
1	Metals/Ions
5	Molecular Weight [Da]
15	Natural Substrates/ Products (Substrates)
42	Organism
1	Oxidation Stability
3	Pathway
3	pH Optimum
19	Protein Variants
7	Purification (Commentary)
6	Reaction
3	Reaction Type
25	Reference
2	Specific Activity [micromol/min/mg]
1	Storage Stability
46	Substrates and Products (Substrate)
6	Subunits
38	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
2	Temperature Range [°C]
1	Temperature Stability [°C]
8	Turnover Number [1/s]

Reaction

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Reaction on EC 1.14.13.242 - 3-hydroxy-2-methylpyridine-5-carboxylate monooxygenase

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REACTION

REACTION DIAGRAM

COMMENTARY

ORGANISM

UNIPROT

LITERATURE

3-hydroxy-2-methylpyridine-5-carboxylate + NAD(P)H + H+ + O2 = 2-(acetamidomethylidene)succinate + NAD(P)+

6 entries

3-hydroxy-2-methylpyridine-5-carboxylate + NAD(P)H + H+ + O2 = 2-(acetamidomethylidene)succinate + NAD(P)+

reaction proceeds in a concerted fashion via a ternary complex of oxygenase, NADH and 3-hydroxy-2-methylpyridine-5-carboxylate

Pseudomonas sp.

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439054

3-hydroxy-2-methylpyridine-5-carboxylate + NAD(P)H + H+ + O2 = 2-(acetamidomethylidene)succinate + NAD(P)+

ordered mechanism in which 3-hydroxy-2-methylpyridine-5-carboxylate binds first, followed by NADH. The first product NAD+ is then released, followed by oxygen binding and finally release of the oxygenated and reduced cleavage product 2-(acetamidomethylene)succinate

Pseudomonas sp.

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439057

3-hydroxy-2-methylpyridine-5-carboxylate + NAD(P)H + H+ + O2 = 2-(acetamidomethylidene)succinate + NAD(P)+

the enzyme catalyzes both a classical hydroxylation and a subsequent unique hydrolysis of the hydroxylated substrate to yield the acyclic product

Pseudomonas sp.

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439061

3-hydroxy-2-methylpyridine-5-carboxylate + NAD(P)H + H+ + O2 = 2-(acetamidomethylidene)succinate + NAD(P)+

the binding proceeds in two steps: an enzyme-substrate complex initially formed is followed by a ligand-induced isomerization

Pseudomonas sp.

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439062

3-hydroxy-2-methylpyridine-5-carboxylate + NAD(P)H + H+ + O2 = 2-(acetamidomethylidene)succinate + NAD(P)+

oxygenation reaction occurs via an electrophilic aromatic substitution mechanism

Pseudomonas sp.

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654745

3-hydroxy-2-methylpyridine-5-carboxylate + NAD(P)H + H+ + O2 = 2-(acetamidomethylidene)succinate + NAD(P)+

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