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1.14.13.24: 3-hydroxybenzoate 6-monooxygenase

This is an abbreviated version!
For detailed information about 3-hydroxybenzoate 6-monooxygenase, go to the full flat file.

Word Map on EC 1.14.13.24

Reaction

3-hydroxybenzoate
+
NADH
+
H+
+
O2
=
2,5-Dihydroxybenzoate
+
NAD+
+
H2O

Synonyms

3-HBA-6-hydroxylase, 3-hydroxybenzoate 6-hydroxylase, 3-hydroxybenzoate-6-hydroxylase, 3-hydroxybenzoic acid-6-hydroxylase, 3HB6H, m-hydroxybenzoate 6-hydroxylase, MNX2, Mnx2p, NagX, NarX, Ncgl2923, oxygenase, 3-hydroxybenzoate 6-mono-, XlnD

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.24 3-hydroxybenzoate 6-monooxygenase

General Stability

General Stability on EC 1.14.13.24 - 3-hydroxybenzoate 6-monooxygenase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
complete inactivation at room temperature without glycerol after 18 h
-
immobilized onto surface-functionalized electrospun polycaprolactone (PCL) fibers can tolerate the changes in temperature and pH better than the free enzyme can. The immobilized enzyme can be reused at least 10 times with more than 60% of the original activity at pH 8 and 25°C. The 3-hydroxybenzoate 6-hydroxylase fibers are potentially useful as a heterogeneous catalyst under the conditions in which free enzyme can not endure.