1.14.13.22: cyclohexanone monooxygenase

This is an abbreviated version!
For detailed information about cyclohexanone monooxygenase, go to the full flat file.

Reaction

Synonyms

Bpro_556, CAMO, CHMO, ChnB, chnB protein, chnB1 protein, CHXON, CMO, cycloalkaone monooxygenase, cyclohexanone 1, 2-mono-oxygenase, cyclohexanone mono-oxygenase, cyclohexanone monooxygenase, cyclohexanone oxygenase, cyclohexanone:NADPH:oxygen oxidoreductase (lactone-forming), oxygenase, cyclohexanone mono-

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

                1.14.13.22 cyclohexanone monooxygenase

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

Results	in table
3	Activating Compound
667	AA Sequence
23	Application
2	CAS Registry Number
33	Cloned(Commentary)
84	Cofactor
5	Crystallization (Commentary)
3	General Information
1	General Stability
37	Inhibitors
30	kcat/KM [mM/s]
11	Ki Value [mM]
102	KM Value [mM]
6	Localization
1	Metals/Ions
16	Molecular Weight [Da]
51	Natural Substrates/ Products (Substrates)
4	Organic Solvent Stability
171	Organism
1	Oxidation Stability
5	Pathway
14	PDB ID
15	pH Optimum
4	pH Range
6	pH Stability
59	Protein Variants
32	Purification (Commentary)
23	Reaction
11	Reaction Type
102	Reference
2	Source Tissue
28	Specific Activity [micromol/min/mg]
10	Storage Stability
491	Substrates and Products (Substrate)
16	Subunits
65	Synonyms
1	Systematic Name
11	Temperature Optimum [°C]
15	Temperature Stability [°C]
72	Turnover Number [1/s]

Turnover Number

print visible entries

print all entries

Turnover Number on EC 1.14.13.22 - cyclohexanone monooxygenase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide show all columns Go to Turnover Number Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

TURNOVER NUMBER [1/s]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.16 - 0.47

2-butanone

12 entries

4.5

2-hydroxycyclobutanone

Ilyonectria destructans

G8H1L8

pH 8.0, 25°C

724071

8.4

2-methylcyclohexyl boronic acid

Acinetobacter sp.

-

-

438975

0.73

2-phenylcyclohexanone

Ilyonectria destructans

G8H1L8

pH 8.0, 25°C

724071

0.1 - 0.4

acetophenone

2 entries

2 - 13.4

bicyclo[3.2.0]hept-2-en-6-one

4 entries

0.683

cis-hex-2-enyl phenyl selenide

Acinetobacter sp.

-

-

438976

2 - 6.8

Cyclobutanone

3 entries

4.3

Cycloheptanone

Ilyonectria destructans

G8H1L8

pH 8.0, 25°C

724071

0.24 - 57.4

cyclohexanone

21 entries

0.68

Cyclooctanone

Ilyonectria destructans

G8H1L8

pH 8.0, 25°C

724071

1 - 5.9

Cyclopentanone

3 entries

0.24

delta-thiovalerolactone

Acinetobacter sp.

-

substrate inactivates enzyme after a few turnovers

438973

0.227

epsilon-thiocaprolactone

Acinetobacter sp.

-

substrate inactivates enzyme after a few turnovers

438973

2.38

ethylene monothiocarbonate

Acinetobacter sp.

-

substrate inactivates enzyme after a few turnovers

438973

0.683

gamma-thiobutyrolactone

Acinetobacter sp.

-

substrate inactivates enzyme after a few turnovers

438973

0.34 - 13.5

NADPH

5 entries

1.11 - 1.31

O2

2 entries

9.75

phenyl propargyl selenide

Acinetobacter sp.

-

-

438976

1

phenylacetone

2 entries

0.35 - 1.12

pyrmetazole

2 entries

-

2 - 3

thioanisole

2 entries

0.917

trans-hex-2-enyl phenyl selenide

Acinetobacter sp.

-

-

438976

additional information

additional information

2 entries

-

0.16

2-butanone

Acinetobacter calcoaceticus

-

pH 7.0, 25°C, mutant enzyme L435N

741489

0.19

2-butanone

Acinetobacter calcoaceticus

-

pH 7.0, 25°C, mutant enzyme I491A

741489

0.2

2-butanone

Acinetobacter calcoaceticus

-

pH 7.0, 25°C, mutant enzyme F281H

741489

0.24

2-butanone

Acinetobacter calcoaceticus

-

pH 7.0, 25°C, mutant enzyme T56S/L244R/I491

741489

0.26

2-butanone

Acinetobacter calcoaceticus

-

pH 7.0, 25°C, mutant enzyme T56S/I491A

741489

0.28

2-butanone

Acinetobacter calcoaceticus

-

pH 7.0, 25°C, wild-type enzyme

741489

0.3

2-butanone

Acinetobacter calcoaceticus

-

pH 7.0, 25°C

741605

0.31

2-butanone

Acinetobacter calcoaceticus

-

pH 7.0, 25°C, mutant enzyme L244R

741489

0.33

2-butanone

Acinetobacter calcoaceticus

-

pH 7.0, 25°C, mutant enzyme T56S/L435N/I491

741489

0.4

2-butanone

Thermocrispum municipale

A0A1L1QK39

pH 7.0, 25°C

741605

0.41

2-butanone

Acinetobacter calcoaceticus

-

pH 7.0, 25°C, mutant enzyme T56S/L244R

741489

0.47

2-butanone

Acinetobacter calcoaceticus

-

pH 7.0, 25°C, mutant enzyme T56S

741489

0.1

acetophenone

Thermocrispum municipale

A0A1L1QK39

pH 7.0, 25°C

741605

0.4

acetophenone

Acinetobacter calcoaceticus

-

pH 7.0, 25°C

741605

2

bicyclo[3.2.0]hept-2-en-6-one

Thermocrispum municipale

A0A1L1QK39

pH 7.0, 25°C

741605

4

bicyclo[3.2.0]hept-2-en-6-one

Acinetobacter calcoaceticus

-

pH 7.0, 25°C

741605

4.9

bicyclo[3.2.0]hept-2-en-6-one

Acinetobacter johnsonii

Q9R2F5

pH 8.5, 20°C, mutant enzyme L323C/A325C

742529

13.4

bicyclo[3.2.0]hept-2-en-6-one

Acinetobacter johnsonii

Q9R2F5

pH 8.5, 20°C, wild-type enzyme

742529

2

Cyclobutanone

Thermocrispum municipale

A0A1L1QK39

pH 7.0, 25°C

741605

4

Cyclobutanone

Acinetobacter calcoaceticus

-

pH 7.0, 25°C

741605

6.8

Cyclobutanone

Ilyonectria destructans

G8H1L8

pH 8.0, 25°C

724071

0.24

cyclohexanone

Acidovorax sp. CHX100

A0A2D0WG32

biofilm, pH not specified in the publication, temperature not specified in the publication

764346

0.43

cyclohexanone

Acinetobacter sp.

P12015

mutant T187A, pH 7.5, 25°C

764018

0.43

cyclohexanone

Acinetobacter sp.

P12015

mutant W490F, pH 7.5, 25°C

764018

1.16

cyclohexanone

Acidovorax sp. CHX100

A0A2D0WG32

purified enzyme, pH not specified in the publication, temperature not specified in the publication

764346

1.5

cyclohexanone

Acidovorax sp. CHX100

A0A2D0WG32

suspended cells, pH not specified in the publication, temperature not specified in the publication

764346

2

cyclohexanone

Thermocrispum municipale

A0A1L1QK39

pH 7.0, 25°C

741605

4.9

cyclohexanone

Rhodococcus sp. HI-31

C0STX7

mutant E91Q/D95H/A115V/R280Y/N431Y/Q439P/A455V/S534R, pH 7.5, temperature not specified in the publication

764343

6

cyclohexanone

Acinetobacter calcoaceticus

-

pH 7.0, 25°C

741605

6.1

cyclohexanone

Acinetobacter johnsonii

Q9R2F5

25°C, pH 8.0, mutant enzyme P254C/D290C

742951

6.1

cyclohexanone

Acinetobacter johnsonii

Q9R2F5

pH 8.5, 20°C, mutant enzyme L323C/A325C

742529

6.15

cyclohexanone

Acinetobacter johnsonii

Q9R2F5

25°C, pH 8.0, mutant enzyme Y411C/A463C

742951

6.5

cyclohexanone

Acinetobacter sp.

P12015

wild-type, pH 7.5, 25°C

764018

9.2

cyclohexanone

Rhodococcus sp. HI-31

C0STX7

wild-type, pH 7.5, temperature not specified in the publication

764343

9.8

cyclohexanone

Ilyonectria destructans

G8H1L8

pH 8.0, 25°C

724071

11.6

cyclohexanone

Acinetobacter sp.

P12015

mutant A245G/A288V, pH 7.7, 25°C

764420

12.9

cyclohexanone

Acinetobacter sp.

P12015

wild-type, pH 7.7, 25°C

764420

14.2

cyclohexanone

Acinetobacter johnsonii

Q9R2F5

pH 8.5, 20°C, wild-type enzyme

742529

30.3

cyclohexanone

Acinetobacter sp.

-

-

438982

38.5

cyclohexanone

Acinetobacter johnsonii

Q9R2F5

25°C, pH 8.0, wild-type enzyme

742951

44.9

cyclohexanone

Acinetobacter johnsonii

Q9R2F5

25°C, pH 8.0, mutant enzyme T415C/A463C

742951

57.4

cyclohexanone

Acinetobacter johnsonii

Q9R2F5

25°C, pH 8.0, mutant enzyme T415C

742951

1

Cyclopentanone

Acinetobacter calcoaceticus

-

pH 7.0, 25°C

741605

2

Cyclopentanone

Thermocrispum municipale

A0A1L1QK39

pH 7.0, 25°C

741605

5.9

Cyclopentanone

Ilyonectria destructans

G8H1L8

pH 8.0, 25°C

724071

0.34

NADPH

Acinetobacter sp.

P12015

recombinant engineered enzyme expressed in Escherichia coli, pH 8.0, temperature not specified in the publication

764312

0.82

NADPH

Acinetobacter sp.

P12015

recombinant engineered enzyme expressed in Pichia pastoris, pH 8.0, temperature not specified in the publication

764312

1.17

NADPH

Acidovorax sp. CHX100

A0A2D0WG32

purified enzyme, pH not specified in the publication, temperature not specified in the publication

764346

9.67

NADPH

Acinetobacter sp.

P12015

mutant A245G/A288V, pH 7.7, 25°C

764420

13.5

NADPH

Acinetobacter sp.

P12015

wild-type, pH 7.7, 25°C

764420

1.11

O2

Acidovorax sp. CHX100

A0A2D0WG32

purified enzyme, pH not specified in the publication, temperature not specified in the publication

764346

1.31

O2

Acidovorax sp. CHX100

A0A2D0WG32

suspended cells, pH not specified in the publication, temperature not specified in the publication

764346

1

phenylacetone

Acinetobacter calcoaceticus

-

pH 7.0, 25°C

741605

1

phenylacetone

Thermocrispum municipale

A0A1L1QK39

pH 7.0, 25°C

741605

0.35

pyrmetazole

Acinetobacter sp.

P12015

recombinant engineered enzyme expressed in Escherichia coli, pH 8.0, temperature not specified in the publication

764312

-

1.12

pyrmetazole

Acinetobacter sp.

P12015

recombinant engineered enzyme expressed in Pichia pastoris, pH 8.0, temperature not specified in the publication

764312

-

2

thioanisole

Thermocrispum municipale

A0A1L1QK39

pH 7.0, 25°C

741605

3

thioanisole

Acinetobacter calcoaceticus

-

pH 7.0, 25°C

741605

additional information

additional information

Acinetobacter sp.

-

-

438975

-

additional information

additional information

Acinetobacter sp.

-

-

438982

-