1.14.13.215: protoasukamycin 4-monooxygenase

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For detailed information about protoasukamycin 4-monooxygenase, go to the full flat file.

Reaction

Synonyms

asuE1, flavin-dependent protoasukamycin hydroxylase, protoasukamycin 4-hydroxylase

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

                1.14.13.215 protoasukamycin 4-monooxygenase

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2	Activating Compound
1	AA Sequence
2	Cloned(Commentary)
8	Cofactor
7	General Information
3	KM Value [mM]
3	Natural Substrates/ Products (Substrates)
3	Organism
1	pH Optimum
1	Purification (Commentary)
1	Reaction
3	Reference
7	Substrates and Products (Substrate)
7	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
3	Turnover Number [1/s]

Cloned

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Cloned on EC 1.14.13.215 - protoasukamycin 4-monooxygenase

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

cloning of asukamycin biosynthetic gene cluster, including gene asuE1 encoding the enzyme, recombinant expression in Escherichia coli strain BW25113/pIJ790, heterologous expression of the complete gene cluster in the asukamycin-nonproducing Streptomyces lividans strain K4-114

Streptomyces nodosus

D7P5V0

736406

gene asuE1, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)

Streptomyces nodosus

D7P5V0

735926