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1.14.13.210: 4-methyl-5-nitrocatechol 5-monooxygenase

This is an abbreviated version!
For detailed information about 4-methyl-5-nitrocatechol 5-monooxygenase, go to the full flat file.

Word Map on EC 1.14.13.210

Reaction

4-methyl-5-nitrocatechol
+
NAD(P)H
+
H+
+
O2
=
2-hydroxy-5-methylquinone
+
nitrite
+
NAD(P)+
+
H2O

Synonyms

4-methyl-5-nitrocatechol monooxygenase, 4-methyl-5-nitrocatechol oxygenase, 4M5NC monooxygenase, dntB, MNC, MNC monooxygenase, MNC oxygenase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.210 4-methyl-5-nitrocatechol 5-monooxygenase

Cloned

Cloned on EC 1.14.13.210 - 4-methyl-5-nitrocatechol 5-monooxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene dntB, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain TG1
gene dntB, sequence comparisons
P71029