1.14.13.2: 4-hydroxybenzoate 3-monooxygenase
This is an abbreviated version!
For detailed information about 4-hydroxybenzoate 3-monooxygenase, go to the full flat file.
Word Map on EC 1.14.13.2
-
1.14.13.2
-
flavin
-
fad
-
fluorescens
-
flavoproteins
-
isoalloxazine
-
protocatechuate
-
3,4-dioxygenase
-
massey
-
2,4-dihydroxybenzoate
-
ballou
-
c4a-hydroperoxide
-
3-hydroxybenzoate
-
gatti
-
degradation
-
analysis
- 1.14.13.2
- flavin
- fad
- fluorescens
- flavoproteins
- isoalloxazine
- protocatechuate
-
3,4-dioxygenase
-
massey
- 2,4-dihydroxybenzoate
-
ballou
-
c4a-hydroperoxide
- 3-hydroxybenzoate
-
gatti
- degradation
- analysis
Reaction
Synonyms
4-HBA 3-hydroxylase, 4-HBA 3-monooxygenase, 4-HBA-3-hydroxylase, 4-HBMO, 4-hydroxybenzoate 3-hydroxylase, 4-hydroxybenzoate 3-monooxygenase, 4-hydroxybenzoate hydroxylase, 4-hydroxybenzoate monooxygenase, 4-hydroxybenzoic hydroxylase, 4HBA 3-hydroxylase, An_PhhA, BxeA2040, FAD-dependent 4-hydroxybenzoate hydroxylase, fungal 4-hydroxybenzoate 3-hydroxylase, HBH, m-hydroxybenzoate hydroxylase, MobA, NADPH-dependent 4-HBA hydroxylase, ncgl1032, oxygenase, 4-hydroxybenzoate 3-mono-, p-hydroxybenzoate hydroxylase, p-hydroxybenzoate-3-hydroxylase, p-hydroxybenzoic acid hydrolase, p-hydroxybenzoic acid hydroxylase, p-hydroxybenzoic hydroxylase, PaPobA, para-hydroxybenzoate hydroxylase, PHBAD, PHBH, PHBHase, PHBHCn2, phhA, PobA, pobACg, POHBase, Reut_B4006
ECTree
1.14.13.2 4-hydroxybenzoate 3-monooxygenaseAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 1.14.13.2 - 4-hydroxybenzoate 3-monooxygenase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2,4-dihydroxybenzoate + NADPH + O2
2,3,4-trihydroxybenzoate + 2,4,5-trihydroxybenzoate + NADP+ + H2O
3,4-dihydroxybenzoic acid + NADPH + H+ + O2
gallic acid + NADP+ + H2O
-
weak activity
-
-
?
3-bromo-4-hydroxybenzoate + NADPH + O2
?
-
3.2% of the activity with 4-hydroxybenzoate
-
-
?
3-Chloro-4-hydroxybenzoate + NADPH + O2
?
-
6.5% of the activity with 4-hydroxybenzoate
-
-
?
3-Fluoro-4-hydroxybenzoate + NADPH + O2
?
-
about 1% of the activity with 4-hydroxybenzoate
-
-
?
4-aminobenzoate + NADPH + O2
?
-
about 1% of the activity with 4-hydroxybenzoate
-
-
?
4-hydroxybenzoate + NADPH + ferricyanide
protocatechuate + NADP+ + ferrocyanide
-
-
-
?
4-toluate + NADPH + O2
?
-
0.29% of the activity with 4-hydroxybenzoate
-
-
?
benzene sulfonate + NADPH + O2
?
-
0.34% of the activity with 4-hydroxybenzoate
-
-
?
m-hydroxybenzoate + NADPH + O2
protocatechuate + NADP+ + H2O
-
-
-
?
p-hydroxybenzoate + NADPH + O2
? + NADP+ + H2O
is transformed to a lesser extent than m-hydroxybenzoate
-
-
?
2,3-dihydroxybenzoate + NADPH + O2
? + NADP+ + H2O
-
-
-
?
2,4-dihydroxybenzoate + NADPH + O2
2,3,4-trihydroxybenzoate + 2,4,5-trihydroxybenzoate + NADP+ + H2O
-
3.1% of the activity with 4-hydroxybenzoate
-
-
?
2,4-dihydroxybenzoate + NADPH + O2
2,3,4-trihydroxybenzoate + 2,4,5-trihydroxybenzoate + NADP+ + H2O
-
-
?
2,4-dihydroxybenzoate + NADPH + O2
2,3,4-trihydroxybenzoate + 2,4,5-trihydroxybenzoate + NADP+ + H2O
-
-
-
-
?
2,4-dihydroxybenzoate + NADPH + O2
2,3,4-trihydroxybenzoate + 2,4,5-trihydroxybenzoate + NADP+ + H2O
-
about 1% of the activity with 4-hydroxybenzoate
-
-
?
2,4-dihydroxybenzoate + NADPH + O2
2,3,4-trihydroxybenzoate + 2,4,5-trihydroxybenzoate + NADP+ + H2O
-
1.5% of the activity with 4-hydroxybenzoate
-
-
?
2,4-dihydroxybenzoate + NADPH + O2
2,3,4-trihydroxybenzoate + 2,4,5-trihydroxybenzoate + NADP+ + H2O
-
slow reaction, formation of at least 3 intermediates, a spectral intermediate that is believed to be an oxygenated form of the enzyme-bound flavin prosthetic group
-
-
?
2,4-dihydroxybenzoate + NADPH + O2
2,3,4-trihydroxybenzoate + 2,4,5-trihydroxybenzoate + NADP+ + H2O
-
8% of the activity with 4-hydroxybenzoate
-
-
?
2,4-dihydroxybenzoate + NADPH + O2
2,3,4-trihydroxybenzoate + 2,4,5-trihydroxybenzoate + NADP+ + H2O
-
8% of the activity with 4-hydroxybenzoate
-
-
?
2,5-dihydroxybenzoate + NADPH + O2
? + NADP+ + H2O
-
-
-
?
2-chloro-4-hydroxybenzoate + NADH + O2
?
-
40% of the activity with 4-hydroxybenzoate
-
-
?
2-chloro-4-hydroxybenzoate + NADH + O2
?
-
40% of the activity with 4-hydroxybenzoate
-
-
?
2-fluoro-4-hydroxybenzoate + NADH + O2
?
-
50% of the activity with 4-hydroxybenzoate
-
-
?
2-fluoro-4-hydroxybenzoate + NADH + O2
?
-
50% of the activity with 4-hydroxybenzoate
-
-
?
3,4-dihydroxybenzoate + NADPH + H+ + O2
gallic acid + NADP+ + H2O
activity of enzyme mutants DA015 and DA016, no activity with the wild-type enzyme
-
-
?
3,4-dihydroxybenzoate + NADPH + H+ + O2
gallic acid + NADP+ + H2O
good substrate of enzyme mutants Y385F and L199V/Y385F, poor activity with the wild-type enzyme PobA
-
-
?
3,4-dihydroxybenzoate + NADPH + H+ + O2
gallic acid + NADP+ + H2O
activity of enzyme mutants DA015 and DA016, no activity with the wild-type enzyme
-
-
?
3,4-dihydroxybenzoate + NADPH + H+ + O2
gallic acid + NADP+ + H2O
good substrate of enzyme mutants Y385F and L199V/Y385F, poor activity with the wild-type enzyme PobA
-
-
?
3,4-dihydroxybenzoate + NADPH + H+ + O2
gallic acid + NADP+ + H2O
activity of enzyme mutants DA015 and DA016, no activity with the wild-type enzyme
-
-
?
3,4-dihydroxybenzoate + NADPH + H+ + O2
gallic acid + NADP+ + H2O
good substrate of enzyme mutants Y385F and L199V/Y385F, poor activity with the wild-type enzyme PobA
-
-
?
3,4-dihydroxybenzoate + NADPH + H+ + O2
gallic acid + NADP+ + H2O
activity of enzyme mutants DA015 and DA016, no activity with the wild-type enzyme
-
-
?
3,4-dihydroxybenzoate + NADPH + H+ + O2
gallic acid + NADP+ + H2O
good substrate of enzyme mutants Y385F and L199V/Y385F, poor activity with the wild-type enzyme PobA
-
-
?
3,4-dihydroxybenzoate + NADPH + H+ + O2
gallic acid + NADP+ + H2O
activity of enzyme mutants DA015 and DA016, no activity with the wild-type enzyme
-
-
?
3,4-dihydroxybenzoate + NADPH + H+ + O2
gallic acid + NADP+ + H2O
good substrate of enzyme mutants Y385F and L199V/Y385F, poor activity with the wild-type enzyme PobA
-
-
?
3,4-dihydroxybenzoate + NADPH + H+ + O2
gallic acid + NADP+ + H2O
activity of enzyme mutants DA015 and DA016, no activity with the wild-type enzyme
-
-
?
3,4-dihydroxybenzoate + NADPH + H+ + O2
gallic acid + NADP+ + H2O
good substrate of enzyme mutants Y385F and L199V/Y385F, poor activity with the wild-type enzyme PobA
-
-
?
3,4-dihydroxybenzoate + NADPH + H+ + O2
gallic acid + NADP+ + H2O
activity of enzyme mutants DA015 and DA016, no activity with the wild-type enzyme
-
-
?
3,4-dihydroxybenzoate + NADPH + H+ + O2
gallic acid + NADP+ + H2O
good substrate of enzyme mutants Y385F and L199V/Y385F, poor activity with the wild-type enzyme PobA
-
-
?
3,4-dihydroxybenzoate + NADPH + H+ + O2
gallic acid + NADP+ + H2O
activity of enzyme mutants DA015 and DA016, no activity with the wild-type enzyme
-
-
?
3,4-dihydroxybenzoate + NADPH + H+ + O2
gallic acid + NADP+ + H2O
good substrate of enzyme mutants Y385F and L199V/Y385F, poor activity with the wild-type enzyme PobA
-
-
?
3,4-dihydroxybenzoate + NADPH + O2
? + NADP+ + H2O
-
-
-
?
3,5-dihydroxybenzoate + NADPH + O2
? + NADP+ + H2O
-
-
-
?
4-hydroxybenzoate + NADH + O2
protocatechuate + NAD+ + H2O
-
the enzyme prefers NADPH to NADH
-
-
?
4-hydroxybenzoate + NADH + O2
protocatechuate + NAD+ + H2O
-
-
-
?
4-hydroxybenzoate + NADH + O2
protocatechuate + NAD+ + H2O
-
-
-
?
4-hydroxybenzoate + NADH + O2
protocatechuate + NAD+ + H2O
-
-
-
?
4-hydroxybenzoate + NADH + O2
protocatechuate + NAD+ + H2O
-
-
-
?
4-hydroxybenzoate + NADPH + H+ + O2
3,4-dihydroxybenzoate + NADP+ + H2O
-
-
-
-
?
4-hydroxybenzoate + NADPH + H+ + O2
3,4-dihydroxybenzoate + NADP+ + H2O
-
-
-
-
?
4-hydroxybenzoate + NADPH + H+ + O2
3,4-dihydroxybenzoate + NADP+ + H2O
-
-
-
?
4-hydroxybenzoate + NADPH + H+ + O2
3,4-dihydroxybenzoate + NADP+ + H2O
-
-
-
?
4-hydroxybenzoate + NADPH + H+ + O2
3,4-dihydroxybenzoate + NADP+ + H2O
-
-
-
?
4-hydroxybenzoate + NADPH + H+ + O2
3,4-dihydroxybenzoate + NADP+ + H2O
-
-
-
?
4-hydroxybenzoate + NADPH + H+ + O2
3,4-dihydroxybenzoate + NADP+ + H2O
-
-
-
?
4-hydroxybenzoate + NADPH + H+ + O2
3,4-dihydroxybenzoate + NADP+ + H2O
-
-
-
?
4-hydroxybenzoate + NADPH + H+ + O2
3,4-dihydroxybenzoate + NADP+ + H2O
-
-
-
?
4-hydroxybenzoate + NADPH + H+ + O2
3,4-dihydroxybenzoate + NADP+ + H2O
-
-
-
?
4-hydroxybenzoate + NADPH + H+ + O2
3,4-dihydroxybenzoate + NADP+ + H2O
-
-
-
?
4-hydroxybenzoate + NADPH + H+ + O2
3,4-dihydroxybenzoate + NADP+ + H2O
-
-
-
ir
4-hydroxybenzoate + NADPH + H+ + O2
3,4-dihydroxybenzoate + NADP+ + H2O
-
-
-
ir
4-hydroxybenzoate + NADPH + H+ + O2
3,4-dihydroxybenzoate + NADP+ + H2O
-
-
-
?
4-hydroxybenzoate + NADPH + H+ + O2
3,4-dihydroxybenzoate + NADP+ + H2O
Pseudomonas putida KT-2440
-
-
-
?
4-hydroxybenzoate + NADPH + H+ + O2
protocatechuate + NADP+ + H2O
-
-
-
-
?
4-hydroxybenzoate + NADPH + H+ + O2
protocatechuate + NADP+ + H2O
-
4-hydroxybenzoate degradation proceeds via hdroxylation to 3,4-dihydroxybenzoic acid and then conversion to catechol, which is cleaved to cis,cis-muconic acid through ortho-catechol cleavage
4-hydroxybenzoate degradation proceeds via hdroxylation to 3,4-dihydroxybenzoic acid and then conversion to catechol, which is cleaved to cis,cis-muconic acid through ortho-catechol cleavage
-
?
4-hydroxybenzoate + NADPH + H+ + O2
protocatechuate + NADP+ + H2O
-
4-hydroxybenzoate degradation proceeds via hdroxylation to 3,4-dihydroxybenzoic acid and then conversion to catechol, which is cleaved to cis,cis-muconic acid through ortho-catechol cleavage
4-hydroxybenzoate degradation proceeds via hdroxylation to 3,4-dihydroxybenzoic acid and then conversion to catechol, which is cleaved to cis,cis-muconic acid through ortho-catechol cleavage
-
?
4-hydroxybenzoate + NADPH + H+ + O2
protocatechuate + NADP+ + H2O
-
-
-
-
?
4-hydroxybenzoate + NADPH + H+ + O2
protocatechuate + NADP+ + H2O
-
-
-
-
?
4-hydroxybenzoate + NADPH + H+ + O2
protocatechuate + NADP+ + H2O
-
-
-
-
?
4-hydroxybenzoate + NADPH + H+ + O2
protocatechuate + NADP+ + H2O
-
-
-
-
?
4-hydroxybenzoate + NADPH + H+ + O2
protocatechuate + NADP+ + H2O
-
-
-
-
?
4-hydroxybenzoate + NADPH + H+ + O2
protocatechuate + NADP+ + H2O
-
-
-
-
?
4-hydroxybenzoate + NADPH + H+ + O2
protocatechuate + NADP+ + H2O
-
-
-
-
?
4-hydroxybenzoate + NADPH + O2
?
-
high degree of homology observed between the enzyme from Comamonas and of Pseudomonas and Acinetobacter indicates the common evolutionary origin of the enzyme in the divergent pathways of 4-hydroxybenzoate among these soil bacteria of different genera
-
-
?
4-hydroxybenzoate + NADPH + O2
?
-
high degree of homology observed between the enzyme from Comamonas and of Pseudomonas and Acinetobacter indicates the common evolutionary origin of the enzyme in the divergent pathways of 4-hydroxybenzoate among these soil bacteria of different genera
-
-
?
4-hydroxybenzoate + NADPH + O2
?
-
high degree of homology observed between the enzyme from Comamonas and of Pseudomonas and Acinetobacter indicates the common evolutionary origin of the enzyme in the divergent pathways of 4-hydroxybenzoate among these soil bacteria of different genera
-
-
?
4-hydroxybenzoate + NADPH + O2
?
-
enzyme is expressed at basal level, presence of 4-hydroxybenzoate enhances activity
-
-
?
4-hydroxybenzoate + NADPH + O2
?
-
inducible enzyme
-
-
?
4-hydroxybenzoate + NADPH + O2
?
-
first step in the bacterial metabolism when 4-hydroxybenzoate is used as growth substrate
-
-
?
4-hydroxybenzoate + NADPH + O2
?
-
enzyme catalyzes an intermediate step in the degradation of aromatic compounds in soil microorganisms
-
-
?
4-hydroxybenzoate + NADPH + O2
?
-
enzyme of the toluene-4-monooxygenase catabolic pathway
-
-
?
4-hydroxybenzoate + NADPH + O2
?
-
enzyme of the toluene-4-monooxygenase catabolic pathway
-
-
?
4-hydroxybenzoate + NADPH + O2
?
-
high degree of homology observed between the enzyme from Comamonas and of Pseudomonas and Acinetobacter indicates the common evolutionary origin of the enzyme in the divergent pathways of 4-hydroxybenzoate among these soil bacteria of different genera
-
-
?
4-hydroxybenzoate + NADPH + O2
protocatechuate + NADP+ + H2O
-
-
-
-
?
4-hydroxybenzoate + NADPH + O2
protocatechuate + NADP+ + H2O
-
enzyme is induced by 4-hydroxybenzoate
-
-
?
4-hydroxybenzoate + NADPH + O2
protocatechuate + NADP+ + H2O
-
-
-
-
?
4-hydroxybenzoate + NADPH + O2
protocatechuate + NADP+ + H2O
-
-
-
-
?
4-hydroxybenzoate + NADPH + O2
protocatechuate + NADP+ + H2O
-
the enzyme prefers NADPH to NADH. 4HBA hydroxylase can be induced by 4HBA, 4-cresol, and 4-hydroxybenzaldehyde
-
-
?
4-hydroxybenzoate + NADPH + O2
protocatechuate + NADP+ + H2O
-
the enzyme prefers NADPH to NADH
-
-
?
4-hydroxybenzoate + NADPH + O2
protocatechuate + NADP+ + H2O
-
-
-
-
?
4-hydroxybenzoate + NADPH + O2
protocatechuate + NADP+ + H2O
-
-
-
-
?
4-hydroxybenzoate + NADPH + O2
protocatechuate + NADP+ + H2O
-
-
-
?
4-hydroxybenzoate + NADPH + O2
protocatechuate + NADP+ + H2O
-
-
-
?
4-hydroxybenzoate + NADPH + O2
protocatechuate + NADP+ + H2O
-
-
-
?
4-hydroxybenzoate + NADPH + O2
protocatechuate + NADP+ + H2O
-
-
390020, 390021, 390022, 390023, 390025, 390027, 390030, 390032, 390033, 390034, 390041, 390042, 390044, 390046, 390049, 686087
-
-
?
4-hydroxybenzoate + NADPH + O2
protocatechuate + NADP+ + H2O
-
-
-
?
4-hydroxybenzoate + NADPH + O2
protocatechuate + NADP+ + H2O
-
at least three intermediates
-
-
?
4-hydroxybenzoate + NADPH + O2
protocatechuate + NADP+ + H2O
-
-
-
-
?
4-hydroxybenzoate + NADPH + O2
protocatechuate + NADP+ + H2O
-
-
-
?
4-hydroxybenzoate + NADPH + O2
protocatechuate + NADP+ + H2O
-
-
-
-
?
4-hydroxybenzoate + NADPH + O2
protocatechuate + NADP+ + H2O
-
-
-
-
?
4-hydroxybenzoate + NADPH + O2
protocatechuate + NADP+ + H2O
-
-
-
?
4-hydroxybenzoate + NADPH + O2
protocatechuate + NADP+ + H2O
-
-
-
-
?
4-hydroxybenzoate + NADPH + O2
protocatechuate + NADP+ + H2O
-
enzyme is induced by 4-hydroxybenzoate
-
-
?
4-hydroxybenzoate + NADPH + O2
protocatechuate + NADP+ + H2O
-
-
-
-
?
4-hydroxybenzoate + NADPH + O2
protocatechuate + NADP+ + H2O
-
-
-
-
?
4-hydroxybenzoate + NADPH + O2
protocatechuate + NADP+ + H2O
-
-
-
-
?
4-hydroxybenzoate + NADPH + O2
protocatechuate + NADP+ + H2O
-
-
-
-
?
4-mercaptobenzoate + NADPH + O2
4,4'-dithiobisbenzoate + ?
-
50% of the activity with 4-hydroxybenzoate
-
?
additional information
?
-
-
the enzyme also shows low activities with 2- and 3-hydroxybenzoate as well as D-glucose
-
-
?
additional information
?
-
-
substrate binding analysis and structure, each subunit of the dimeric enzyme contains a split substrate-binding domain, overview
-
-
-
additional information
?
-
-
substrate binding analysis and structure, each subunit of the dimeric enzyme contains a split substrate-binding domain, overview
-
-
-
additional information
?
-
-
p-hydroxybenzoate induces the expression of p-hydroxybenzoate hydroxylase
-
-
?
additional information
?
-
is not capable of hydroxylating benzoate, o-hydroxybenzoate (salicylate), 2,4-dihydroxybenzoate, 2,6-dihydroxybenzoate, 2-chlorophenol, 3-aminophenol, 4-methoxybenzoate, 3-toluate, o-cresol, m-cresol, or p-cresol
-
-
?
additional information
?
-
is not capable of hydroxylating benzoate, o-hydroxybenzoate (salicylate), 2,4-dihydroxybenzoate, 2,6-dihydroxybenzoate, 2-chlorophenol, 3-aminophenol, 4-methoxybenzoate, 3-toluate, o-cresol, m-cresol, or p-cresol
-
-
?
additional information
?
-
-
is not capable of hydroxylating benzoate, o-hydroxybenzoate (salicylate), 2,4-dihydroxybenzoate, 2,6-dihydroxybenzoate, 2-chlorophenol, 3-aminophenol, 4-methoxybenzoate, 3-toluate, o-cresol, m-cresol, or p-cresol
-
-
?
additional information
?
-
-
no hydroxylase activity is observed with 3-hydroxybenzoate, resorcinol, 3-nitrophenol, benzoate, 2,4-dihydroxybenzoate, 4-chlorobenzoate, 4-aminobenzoate, 4-aminophenol, 4-nitrophenol, and 4-dinitrobenzene
-
-
?
additional information
?
-
-
a Tyr seems to be involved in substrate activation
-
-
?
additional information
?
-
-
mutant enzyme Y385F hydroxylates 3,4-dihydroxybenzoate to form gallic acid
-
-
?
additional information
?
-
3,4-dihydroxybenzoate is no substrate for hydroxylation by the enzyme. But PHBH can bind to other benzoate derivatives in addition to 4-hydroxybenzoate
-
-
-
additional information
?
-
3,4-dihydroxybenzoate is no substrate for hydroxylation by the enzyme. But PHBH can bind to other benzoate derivatives in addition to 4-hydroxybenzoate
-
-
-
additional information
?
-
3,4-dihydroxybenzoate is no substrate for hydroxylation by the enzyme. But PHBH can bind to other benzoate derivatives in addition to 4-hydroxybenzoate
-
-
-
additional information
?
-
3,4-dihydroxybenzoate is no substrate for hydroxylation by the enzyme. But PHBH can bind to other benzoate derivatives in addition to 4-hydroxybenzoate
-
-
-
additional information
?
-
3,4-dihydroxybenzoate is no substrate for hydroxylation by the enzyme. But PHBH can bind to other benzoate derivatives in addition to 4-hydroxybenzoate
-
-
-
additional information
?
-
3,4-dihydroxybenzoate is no substrate for hydroxylation by the enzyme. But PHBH can bind to other benzoate derivatives in addition to 4-hydroxybenzoate
-
-
-
additional information
?
-
3,4-dihydroxybenzoate is no substrate for hydroxylation by the enzyme. But PHBH can bind to other benzoate derivatives in addition to 4-hydroxybenzoate
-
-
-
additional information
?
-
3,4-dihydroxybenzoate is no substrate for hydroxylation by the enzyme. But PHBH can bind to other benzoate derivatives in addition to 4-hydroxybenzoate
-
-
-
additional information
?
-
-
Arg42 is involved in binding of the 2'-phosphoadenosine moiety of NADPH
-
-
?
additional information
?
-
-
under anaerobic conditions, the enzyme can catalyze a reduction of FAD by NADPH provided that 4-hydroxybenzoate is present
-
-
?
additional information
?
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development of a colorimetric coupled assay: the glucose-6-phosphate dehydrogenase (G6PD) catalyzes the specific dehydrogenation of glucose-6-phosphate (G-6-P) by consuming the oxidized form of beta-nicotinamide adenine dinucleotide phosphate (NADP+) to generate NADPH. And the formed NADPH initiates the irreversible hydroxylation of p-hydroxybenzoate by p-hydroxybenzoate hydroxylase (PHBH) to generate 3,4-dihydroxybenzoate (DHB), which results in the subsequent in situ generation of a photoresponsive nanozyme of DHB coordinated SrTiO3 (SrTiO3/DHB) nanosheets. The photoresponsive nanozyme has intriguing interfacial charge transfer (ICT) characteristics and thus realizes the efficiently catalytic oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) under light irradiation for signal readout. Optimization and evaluation of the ultrasensitive colorimetric bioassay for versatile targets, overview
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additional information
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development of a colorimetric coupled assay: the glucose-6-phosphate dehydrogenase (G6PD) catalyzes the specific dehydrogenation of glucose-6-phosphate (G-6-P) by consuming the oxidized form of beta-nicotinamide adenine dinucleotide phosphate (NADP+) to generate NADPH. And the formed NADPH initiates the irreversible hydroxylation of p-hydroxybenzoate by p-hydroxybenzoate hydroxylase (PHBH) to generate 3,4-dihydroxybenzoate (DHB), which results in the subsequent in situ generation of a photoresponsive nanozyme of DHB coordinated SrTiO3 (SrTiO3/DHB) nanosheets. The photoresponsive nanozyme has intriguing interfacial charge transfer (ICT) characteristics and thus realizes the efficiently catalytic oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) under light irradiation for signal readout. Optimization and evaluation of the ultrasensitive colorimetric bioassay for versatile targets, overview
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additional information
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comparisons of substrate binding structure analysis mechanism
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additional information
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comparisons of substrate binding structure analysis mechanism
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additional information
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Pseudomonas putida KT-2440
comparisons of substrate binding structure analysis mechanism
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additional information
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the enzyme also shows low activities with 2- and 3-hydroxybenzoate as well as D-glucose
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additional information
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no activity with 2-hydroxybenzoate and 3-hydroxybenzoate
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additional information
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no activity with 2-hydroxybenzoate and 3-hydroxybenzoate
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