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1.14.13.2: 4-hydroxybenzoate 3-monooxygenase

This is an abbreviated version!
For detailed information about 4-hydroxybenzoate 3-monooxygenase, go to the full flat file.

Word Map on EC 1.14.13.2

Reaction

4-hydroxybenzoate
+
NADPH
 +
H+
 +
O2
=
3,4-dihydroxybenzoate
 +
NADP+
 +
H2O

Synonyms

4-HBA 3-hydroxylase, 4-HBA 3-monooxygenase, 4-HBA-3-hydroxylase, 4-HBMO, 4-hydroxybenzoate 3-hydroxylase, 4-hydroxybenzoate 3-monooxygenase, 4-hydroxybenzoate hydroxylase, 4-hydroxybenzoate monooxygenase, 4-hydroxybenzoic hydroxylase, 4HBA 3-hydroxylase, An_PhhA, BxeA2040, FAD-dependent 4-hydroxybenzoate hydroxylase, fungal 4-hydroxybenzoate 3-hydroxylase, HBH, m-hydroxybenzoate hydroxylase, MobA, NADPH-dependent 4-HBA hydroxylase, ncgl1032, oxygenase, 4-hydroxybenzoate 3-mono-, p-hydroxybenzoate hydroxylase, p-hydroxybenzoate-3-hydroxylase, p-hydroxybenzoic acid hydrolase, p-hydroxybenzoic acid hydroxylase, p-hydroxybenzoic hydroxylase, PaPobA, para-hydroxybenzoate hydroxylase, PHBAD, PHBH, PHBHase, PHBHCn2, phhA, PobA, pobACg, POHBase, Reut_B4006

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.2 4-hydroxybenzoate 3-monooxygenase

Cloned

Cloned on EC 1.14.13.2 - 4-hydroxybenzoate 3-monooxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
expressed in Escherichia coli BL21 Star (DE3) cells
-
expressed in Escherichia coli TG2 cells
-
expression in Escherichia coli
-
expression in Escherichia coli, mutant enzyme S212A
expression of mutant enzymes Y201F and Y385F in Escherichia coli
-
expression of wild-type and E49Q mutant PHPH in Escherichia coli
-
gene phhA, sequence comparisons and phylogenetic analysis and tree
-
gene pobA, cloned into the ampicillin-resistant pMCSG53 vector, which contains an N-terminal polyhistidine tag followed by the Tobacco etch virus (TEV) protease cleavage site and the start codon of the pobA gene, recombinant expression in kanamycin-resistant Escherichia coli strain BL21(DE3)
gene pobA, construction and selection of PobA library, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21 (DE3)
gene Reut_B4006, sequence comparisons and phylogenetic analysis and tree, recombinant expression of the codon-optimized gene in Acinetobacter sp. ADP1
mobA gene from pSEB2 cloned into pZR80. Random mutations introduced into the mobA gene and cloned into pCR2.1-TOPO and transformed into Escherichia coli Top10
mutant enzymes K297M, N300D and Y385F
-
mutant enzymes R42S and R42K expressed in transformed Escherichia coli TG2 cells
-
overexpression in Escherichia coli
-
plasmid mutagenesis for high-level expression of 4-hydroxybenzoate hydroxylase
-
When expressed in Escherichia coli, p-hydroxybenzoate hydroxylase transforms p-hydroxybenzoate into protocatechuate
-