1.14.13.195: L-ornithine N5-monooxygenase (NADPH)
This is an abbreviated version!
For detailed information about L-ornithine N5-monooxygenase (NADPH), go to the full flat file.
Word Map on EC 1.14.13.195
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1.14.13.195
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flavin
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fumigatus
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aspergillus
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flavin-dependent
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pyoverdine
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nadp+
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c4a-hydroperoxyflavin
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hydroxamate
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hydroxamate-containing
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flavin-containing
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non-ribosomal
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half-reaction
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n-hydroxylating
- 1.14.13.195
- flavin
- fumigatus
- aspergillus
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flavin-dependent
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pyoverdine
- nadp+
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c4a-hydroperoxyflavin
- hydroxamate
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hydroxamate-containing
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flavin-containing
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non-ribosomal
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half-reaction
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n-hydroxylating
Reaction
Synonyms
Af-OMO, CchB, flavin-dependent monooxygenase, L-Orn N5-oxygenase, L-ornithine N5-hydroxylase, L-ornithine-Ndelta-oxygenase, monooxygenase EtcB, omega-amino acid monooxygenase, ornithine hydroxylase, ornithine N5-monooxygenase, PsbA, PvdA, SidA, siderophore A
ECTree
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Reaction
Reaction on EC 1.14.13.195 - L-ornithine N5-monooxygenase (NADPH)
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L-ornithine + NADPH + H+ + O2 = N5-hydroxy-L-ornithine + NADP+ + H2O
binding of the hydroxylation target is not required to trigger reduction of flavin by NADPH, the reductive half-reaction proceeds in presence and absence of ornithine, reaction of O2 with FADH2 is accelerated 80fold by ornithine, ensuring coupling of NADPH and ornithine, C(4a)-hydroperoxyflavin intermediate, overview
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L-ornithine + NADPH + H+ + O2 = N5-hydroxy-L-ornithine + NADP+ + H2O
sequential kinetic mechanism for SidA. The reaction is initiated by binding of NADPH to SidA with the flavin in its oxidized form (FADox). The rate-limiting step in the reaction is the stereospecific transfer of the pro-R-hydride equivalent from NADPH to yield reduced flavin (FADred) and NADP+. The FADred-NADP+ complex reacts with molecular oxygen forming the C4a-hydroperoxyflavin (FADOOH). In this complex, NADP+ is essential for stabilization of the FADOOH. After ornithine (Orn) binding, hydroxylation occurs very quickly. The last step in the reaction is the release of products