1.14.13.195: L-ornithine N5-monooxygenase (NADPH)
This is an abbreviated version!
For detailed information about L-ornithine N5-monooxygenase (NADPH), go to the full flat file.
Word Map on EC 1.14.13.195
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1.14.13.195
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flavin
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fumigatus
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aspergillus
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flavin-dependent
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pyoverdine
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nadp+
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c4a-hydroperoxyflavin
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hydroxamate
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hydroxamate-containing
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flavin-containing
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non-ribosomal
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half-reaction
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n-hydroxylating
- 1.14.13.195
- flavin
- fumigatus
- aspergillus
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flavin-dependent
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pyoverdine
- nadp+
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c4a-hydroperoxyflavin
- hydroxamate
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hydroxamate-containing
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flavin-containing
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non-ribosomal
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half-reaction
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n-hydroxylating
Reaction
Synonyms
Af-OMO, CchB, flavin-dependent monooxygenase, L-Orn N5-oxygenase, L-ornithine N5-hydroxylase, L-ornithine-Ndelta-oxygenase, monooxygenase EtcB, omega-amino acid monooxygenase, ornithine hydroxylase, ornithine N5-monooxygenase, PsbA, PvdA, SidA, siderophore A
ECTree
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KM Value
KM Value on EC 1.14.13.195 - L-ornithine N5-monooxygenase (NADPH)
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1
L-ornithine
pH 7.5, 25°C, recombinant wild-type enzyme, ornithine hydroxylation assay
1.1
L-ornithine
pH 7.5, 25°C, recombinant wild-type enzyme, O2 consumption assay
12
L-ornithine
pH 7.5, 25°C, recombinant mutant N323A, ornithine hydroxylation assay
15
L-ornithine
pH 7.5, 25°C, recombinant mutant N323A, O2 consumption assay
16
L-ornithine
pH 7.5, 25°C, recombinant mutant N293A, ornithine hydroxylation assay
18
L-ornithine
pH 7.5, 25°C, recombinant mutant N293A, O2 consumption assay
18
L-ornithine
pH 7.5, 25°C, recombinant mutant S469A, ornithine hydroxylation assay
21
L-ornithine
pH 7.5, 25°C, recombinant mutant S469A, O2 consumption assay
0.007
pH 7.5, 25°C, recombinant wild-type enzyme, O2 consumption assay
0.01
NADPH
pH 7.5, 25°C, recombinant mutant N323A, O2 consumption assay
0.025
NADPH
pH 7.5, 25°C, recombinant mutant S469A, O2 consumption assay
0.06
NADPH
pH 7.5, 25°C, recombinant mutant N293A, O2 consumption assay
0.34
NADPH
Q5SE95
mutant S257A, hydroxylamine detection assay, pH 7.5, 25°C
0.8
NADPH
Q5SE95
wild-type, hydroxylamine detection assay, pH 7.5, 25°C
additional information
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Michaelis-Menten kinetics
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additional information
additional information
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Michaelis-Menten kinetics
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additional information
additional information
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kinetic mechanism of oxidative and reductive half-reactions, stopped-flow kinetics
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additional information
additional information
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Michaelis-Menten steady-state kinetic analysis, overview
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additional information
additional information
Pre-steady-state kinetics and steady-state kinetics
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additional information
additional information
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Pre-steady-state kinetics and steady-state kinetics
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