1.14.13.181: 13-deoxydaunorubicin hydroxylase

This is an abbreviated version!
For detailed information about 13-deoxydaunorubicin hydroxylase, go to the full flat file.

Reaction

Synonyms

CYP109A2, DoxA, DoxA monooxygenase

ECTree

1 Oxidoreductases

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

1.14.13.181 13-deoxydaunorubicin hydroxylase

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Results	in table
5	AA Sequence
3	Cloned(Commentary)
6	Cofactor
6	General Information
6	Inhibitors
4	kcat/KM [mM/s]
1	Ki Value [mM]
4	KM Value [mM]
4	Molecular Weight [Da]
13	Natural Substrates/ Products (Substrates)
10	Organism
6	Pathway
1	pH Optimum
2	Purification (Commentary)
2	Reaction
9	Reference
1	Source Tissue
1	Specific Activity [micromol/min/mg]
1	Storage Stability
25	Substrates and Products (Substrate)
5	Subunits
11	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
1	Temperature Stability [°C]
4	Turnover Number [1/s]

Purification

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Purification on EC 1.14.13.181 - 13-deoxydaunorubicin hydroxylase

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recombinant His-tagged enzyme DoxA from Escherichia coli, copurification with His-tagged FDX1, FDR2, FDX3, and putidaredoxin and putidaredoxin reductase from Pseudomonas putida from Escherichia coli by cobalt affinity chromatography and ultrafiltration

Streptomyces peucetius

744164

the recombinant DoxA is purified to homogeneity from Streptomyces lividans transformed with a plasmid containing the Streptomyces sp. strain C5 doxA gene under the control of the strong SnpR-activated snpA promoter

Streptomyces sp.

Q59971

722500