1.14.13.166: 4-nitrocatechol 4-monooxygenase

This is an abbreviated version!
For detailed information about 4-nitrocatechol 4-monooxygenase, go to the full flat file.

Word Map on EC 1.14.13.166

Reaction

4-nitrocatechol
+
NAD(P)H
+
H+
+
O2
=
2-hydroxy-1,4-benzoquinone
+
nitrite
+
NAD(P)+
+
H2O

Synonyms

4-nitrophenol 4-monooxygenase/4-nitrocatechol 2-monooxygenase, NpcA, npcB, NpdA2, p-nitrophenol monooxygenase, p-nitrophenol monooxygenase-II, para-nitrophenol 4-monooxygenase, PdcA, pentachlorophenol-4-monooxygenase, PNP 4-monooxygenase, PNP monooxygenase, pnpA, two-component PNP monooxygenase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.166 4-nitrocatechol 4-monooxygenase

Storage Stability

Storage Stability on EC 1.14.13.166 - 4-nitrocatechol 4-monooxygenase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, without glycerol, rapid inactivation
-70C, in 50% (v/v) glycerol, 7 days, 70% loss of activity
0C, extracts prepared without added FAD, 48 h, 52% loss of activity
-
0C, storage medium, no loss of activity after several hours, 30% loss of activity after 12 h, 80% loss of activity after 40 h