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1.14.13.161: (+)-camphor 6-exo-hydroxylase

This is an abbreviated version!
For detailed information about (+)-camphor 6-exo-hydroxylase, go to the full flat file.

Reaction

(+)-Camphor
+
NADPH
+
H+
+
O2
=
(+)-6-exo-hydroxycamphor
+
NADP+
+
H2O

Synonyms

(+)-camphor 6-hydroxylase, P450cam

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.161 (+)-camphor 6-exo-hydroxylase

Engineering

Engineering on EC 1.14.13.161 - (+)-camphor 6-exo-hydroxylase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
functional expression in Escherichia coli of tricistronic constructs consisting of P450cam and the auxiliary proteins, Pd and PdR. Transformed bacterial whole cells efficiently oxidize (1R)-(+)-camphor to 6-exo-hydroxycamphor and interestingly limonene to (-)-perillyl alcohol. These bioengineered Escherichia coli cells possess a heterologous selfsufficient P450 catalytic system