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1.14.13.154: erythromycin 12-hydroxylase

This is an abbreviated version!
For detailed information about erythromycin 12-hydroxylase, go to the full flat file.

Wordmap for 1.14.13.154

Word Map on EC 1.14.13.154 Wordmap for 1.14.13.154

Reaction

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erythromycin D
+
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NADPH
+
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H+
+
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O2
=
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erythromycin C
+
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NADP+
+
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H2O

Synonyms

CYP113A1, EryK, erythromycin C-12 hydroxylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                EC 1.14.13.1541.14.13.154 erythromycin 12-hydroxylase

Crystallization

Crystallization on EC 1.14.13.154 - erythromycin 12-hydroxylase

for references in articles please use BRENDA:EC1.14.13.154

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystal structures of EryK in complex with the azole inhibitors ketoconazole and clotrimazole
different crystal forms are harvested from distinct crystallization conditions: two ligand-free forms, one substrate bound and two inhibitors-bound. All crystals belong either to the monoclinc P2(1)or to the orthorhombic P2(1)2(1)2(1) space groups, and exhibit diffraction limits ranging from 2.3 to 1.6 A
vapor diffusion at 21°C, three crystal forms of EryK are obtained in different crystallization conditions: with His tag (His6-EryK) in low salt conditions, without tag (EryK) in high salt, and in complex with its substrate erythromycin D (ErD-EryK)