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BRENDA support

1.14.13.154: erythromycin 12-hydroxylase

This is an abbreviated version!
For detailed information about erythromycin 12-hydroxylase, go to the full flat file.

Wordmap for 1.14.13.154

Word Map on EC 1.14.13.154 Wordmap for 1.14.13.154

Reaction

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erythromycin D
+
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NADPH
+
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H+
+
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O2
=
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erythromycin C
+
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NADP+
+
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H2O

Synonyms

CYP113A1, EryK, erythromycin C-12 hydroxylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                EC 1.14.13.1541.14.13.154 erythromycin 12-hydroxylase

Cloned

Cloned on EC 1.14.13.154 - erythromycin 12-hydroxylase

for references in articles please use BRENDA:EC1.14.13.154

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
overproduction of EryK in Escherichia coli as insoluble inclusion bodies
-
recombinant His-tagged EryK is overexpressed in BL21 STAR (DE3) Escherichia coli strain
recombinant His-tagged EryK is overexpressed in the Escherichia coli BL21